{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(1)"],"submitter":["Timmermans RGM"],"pubmed_abstract":["<h4>Objective</h4>Due to the complexity and heterogeneity of osteoarthritis (OA) pathophysiology, studying the interaction between intrinsic molecular changes in chondrocytes after hyper-physiological mechanical stress (MS) and aberrant signalling of OA risk genes remains a challenge. In this study we set out to set up an <i>in vitro</i> 3D neo cartilage pellet model that enables us to explore the responses of OA risk genes to hyper-physiological MS.<h4>Design</h4>Human primary chondrocyte neo-cartilage pellets were exposed for 2 days to 2 ​× ​10 ​min of hyper-physiological dynamic MS attained by a 20% strain and a frequency of 5 ​Hz. In order to assess cartilage damage, sulphated glycosaminoglycan (sGAG) content in the neo-cartilage was quantified using Alcian blue staining and a dimethyl"],"journal":["Osteoarthritis and cartilage open"],"pagination":["100231"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718246"],"repository":["biostudies-literature"],"pubmed_title":["A human <i>in vitro</i> 3D neo-cartilage model to explore the response of OA risk genes to hyper-physiological mechanical stress."],"pmcid":["PMC9718246"],"pubmed_authors":["Tuerlings M","Ramos YFM","Timmermans RGM","Bloks NGC","Meulenbelt I","van Hoolwerff M","van der Wal RJP","van den Bosch MHJ","Nelissen RGHH","Blom AB","van der Kraan PM"],"additional_accession":[]},"is_claimable":false,"name":"A human <i>in vitro</i> 3D neo-cartilage model to explore the response of OA risk genes to hyper-physiological mechanical stress.","description":"<h4>Objective</h4>Due to the complexity and heterogeneity of osteoarthritis (OA) pathophysiology, studying the interaction between intrinsic molecular changes in chondrocytes after hyper-physiological mechanical stress (MS) and aberrant signalling of OA risk genes remains a challenge. In this study we set out to set up an <i>in vitro</i> 3D neo cartilage pellet model that enables us to explore the responses of OA risk genes to hyper-physiological MS.<h4>Design</h4>Human primary chondrocyte neo-cartilage pellets were exposed for 2 days to 2 ​× ​10 ​min of hyper-physiological dynamic MS attained by a 20% strain and a frequency of 5 ​Hz. In order to assess cartilage damage, sulphated glycosaminoglycan (sGAG) content in the neo-cartilage was quantified using Alcian blue staining and a dimethyl","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-29T04:34:49.395Z","creation":"2025-04-06T14:07:24.781Z"},"accession":"S-EPMC9718246","cross_references":{"pubmed":["36474468"],"doi":["10.1016/j.ocarto.2021.100231"]}}