biostudies-literatureBera AScience and Engineering Research BoardCouncil of Scientific and Industrial ResearchDepartment of Biotechnology , Ministry of Science and TechnologyDepartment of Science and Technology, Ministry of Science and Technology171-178https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718314biostudies-literatureUnknown2(3)We demonstrate experimental evidence of the effect of surface plasmon resonance of noble metal nanoparticles (NPs) on the activity of a well-known biomedicinal drug in the proximity of a semiconductor having a wide band gap for enhanced photodynamic therapy (PDT) efficacy. We have chosen riboflavin (Rf) (or vitamin B₂) as a model photosensitizer, attached with ZnO NPs and further attached with gold (Au) NP-decorated ZnO to increase the efficiency. The synthesized nanohybrids are characterized with the help of different microscopic, optical spectroscopic, and density functional theory (DFT)-based techniques. The DFT and time-dependent DFT-based calculations validate the experimental findings. A detailed ultrafast spectroscopic study has been carried out further to study the excitACS physical chemistry AuDual Sensitization via Electron and Energy Harvesting in a Nanohybrid for Improvement of Therapeutic Efficacy.PMC9718314DBT-BT/PR11534/NNT/28/766/2014EMR/2016/ 004698INAE/121/ AKFPal SKBera AChatterjee AHasan MNMukherjee DfalseDual Sensitization via Electron and Energy Harvesting in a Nanohybrid for Improvement of Therapeutic Efficacy.We demonstrate experimental evidence of the effect of surface plasmon resonance of noble metal nanoparticles (NPs) on the activity of a well-known biomedicinal drug in the proximity of a semiconductor having a wide band gap for enhanced photodynamic therapy (PDT) efficacy. We have chosen riboflavin (Rf) (or vitamin B₂) as a model photosensitizer, attached with ZnO NPs and further attached with gold (Au) NP-decorated ZnO to increase the efficiency. The synthesized nanohybrids are characterized with the help of different microscopic, optical spectroscopic, and density functional theory (DFT)-based techniques. The DFT and time-dependent DFT-based calculations validate the experimental findings. A detailed ultrafast spectroscopic study has been carried out further to study the excit2022-01-01T00:00:00Z2022 May2026-05-29T20:17:22.938Z2025-04-07T00:31:45.971ZS-EPMC97183143685557110.1021/acsphyschemau.1c00033