biostudies-literatureWheeler NJNIAID NIH HHSDivision of Intramural Research, National Institute of Allergy and Infectious Diseasese0010937https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718391biostudies-literatureUnknown16(11)Advances in high-throughput and high-content imaging technologies require concomitant development of analytical software capable of handling large datasets and generating relevant phenotypic measurements. Several tools have been developed to analyze drug response phenotypes in parasitic and free-living worms, but these are siloed and often limited to specific instrumentation, worm species, and single phenotypes. No unified tool exists to analyze diverse high-content phenotypic imaging data of worms and provide a platform for future extensibility. We have developed wrmXpress, a unified framework for analyzing a variety of phenotypes matched to high-content experimental assays of free-living and parasitic nematodes and flatworms. We demonstrate its utility for analyzing a suite of phenotypesPLoS neglected tropical diseaseswrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms.PMC9718391F32 AI152347R01 AI151171HHSN272201700014CR21 AI153545Zamanian MRyan KTWheeler NJGallo KJChan JDRehborg EJGfalsewrmXpress: A modular package for high-throughput image analysis of parasitic and free-living worms.Advances in high-throughput and high-content imaging technologies require concomitant development of analytical software capable of handling large datasets and generating relevant phenotypic measurements. Several tools have been developed to analyze drug response phenotypes in parasitic and free-living worms, but these are siloed and often limited to specific instrumentation, worm species, and single phenotypes. No unified tool exists to analyze diverse high-content phenotypic imaging data of worms and provide a platform for future extensibility. We have developed wrmXpress, a unified framework for analyzing a variety of phenotypes matched to high-content experimental assays of free-living and parasitic nematodes and flatworms. We demonstrate its utility for analyzing a suite of phenotypes2022-01-01T00:00:00Z2022 Nov2026-05-28T01:10:33.628Z2025-04-19T22:47:33.724ZS-EPMC97183913639949110.1371/journal.pntd.0010937