biostudies-literatureUnknown13Huang J<h4>Objective</h4>Thyroid-associated ophthalmopathy (TAO) is a disfiguring autoimmune disease, which destroys the structure of orbital tissues and even threatens vision. Metabolic reprograming is critical in autoimmune diseases; however, the metabolic basis of TAO remains to be clarified. Our study aimed to reveal the metabolic profile of TAO.<h4>Methods</h4>Orbital adipose/connective tissues from eleven TAO patients and twelve control subjects were collected during surgeries and analyzed with liquid chromatograph-mass spectrometer. Orthogonal partial least-squares discrimination analysis (OPLS-DA), variable importance in projection (VIP), heat map, and volcano plot were used to reveal metabolic profile in TAO. Pathway analysis and metabolites-gene analysis were utilized to explore potentiFrontiers in endocrinology1001349https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718489biostudies-literatureIntegrative metabolic analysis of orbital adipose/connective tissue in patients with thyroid-associated ophthalmopathy.PMC9718489Wu LHu YZhao CHuang JChen MLiang YZhang YXia WfalseIntegrative metabolic analysis of orbital adipose/connective tissue in patients with thyroid-associated ophthalmopathy.<h4>Objective</h4>Thyroid-associated ophthalmopathy (TAO) is a disfiguring autoimmune disease, which destroys the structure of orbital tissues and even threatens vision. Metabolic reprograming is critical in autoimmune diseases; however, the metabolic basis of TAO remains to be clarified. Our study aimed to reveal the metabolic profile of TAO.<h4>Methods</h4>Orbital adipose/connective tissues from eleven TAO patients and twelve control subjects were collected during surgeries and analyzed with liquid chromatograph-mass spectrometer. Orthogonal partial least-squares discrimination analysis (OPLS-DA), variable importance in projection (VIP), heat map, and volcano plot were used to reveal metabolic profile in TAO. Pathway analysis and metabolites-gene analysis were utilized to explore potenti2022-01-01T00:00:00Z20222026-06-04T09:27:22.515Z2025-05-29T16:36:36.188ZS-EPMC97184893646565810.3389/fendo.2022.1001349