biostudies-literatureSherpa DDeutsche ForschungsgemeinschaftMax-Planck-Gesellschafte77937https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718529biostudies-literatureUnknown11The development of haematopoietic stem cells into mature erythrocytes - erythropoiesis - is a controlled process characterized by cellular reorganization and drastic reshaping of the proteome landscape. Failure of ordered erythropoiesis is associated with anaemias and haematological malignancies. Although the ubiquitin system is a known crucial post-translational regulator in erythropoiesis, how the erythrocyte is reshaped by the ubiquitin system is poorly understood. By measuring the proteomic landscape of in vitro human erythropoiesis models, we found dynamic differential expression of subunits of the CTLH E3 ubiquitin ligase complex that formed maturation stage-dependent assemblies of topologically homologous RANBP9- and RANBP10-CTLH complexes. Moreover, protein abundance of CTLH's cogneLifeModular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation.PMC9718529SCHU3196/1-1Nilvebrant JXu PChrustowicz JSidhu SSMurray PJKarayel OSherpa DCzarnecki OMann MGross AZhang WYao YWeiss MJMueller JBaumann CSchulman BAGu JAlpi AFGottemukkala KVfalseModular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation.The development of haematopoietic stem cells into mature erythrocytes - erythropoiesis - is a controlled process characterized by cellular reorganization and drastic reshaping of the proteome landscape. Failure of ordered erythropoiesis is associated with anaemias and haematological malignancies. Although the ubiquitin system is a known crucial post-translational regulator in erythropoiesis, how the erythrocyte is reshaped by the ubiquitin system is poorly understood. By measuring the proteomic landscape of in vitro human erythropoiesis models, we found dynamic differential expression of subunits of the CTLH E3 ubiquitin ligase complex that formed maturation stage-dependent assemblies of topologically homologous RANBP9- and RANBP10-CTLH complexes. Moreover, protein abundance of CTLH's cogn2022-01-01T00:00:00Z2022 Dec2026-05-28T01:10:39.086Z2025-04-19T22:47:50.982ZS-EPMC97185293645948410.7554/eLife.77937