{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McAffee DB"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","Novo Nordisk Fonden"],"pagination":["7446"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718779"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["LAT assembly into a two-dimensional protein condensate is a prominent feature of antigen discrimination by T cells. Here, we use single-molecule imaging techniques to resolve the spatial position and temporal duration of each pMHC:TCR molecular binding event while simultaneously monitoring LAT condensation at the membrane. An individual binding event is sufficient to trigger a LAT condensate, which is self-limiting, and neither its size nor lifetime is correlated with the duration of the originating pMHC:TCR binding event. Only the probability of the LAT condensate forming is related to the pMHC:TCR binding dwell time. LAT condenses abruptly, but after an extended delay from the originating binding event. A LAT mutation that facilitates phosphorylation at the PLC-γ1 recruitment site shortens the delay time to LAT condensation and alters T cell antigen specificity. These results identify a function for the LAT protein condensation phase transition in setting antigen discrimination thresholds in T cells."],"journal":["Nature communications"],"pubmed_title":["Discrete LAT condensates encode antigen information from single pMHC:TCR binding events."],"pmcid":["PMC9718779"],"funding_grant_id":["NNF17OC0028176","AI091580","P01 AI091580"],"pubmed_authors":["Low-Nam ST","Kim S","McAffee DB","Wilhelm KB","O'Dair MK","Lin JJ","Morita S","Groves JT"],"additional_accession":[]},"is_claimable":false,"name":"Discrete LAT condensates encode antigen information from single pMHC:TCR binding events.","description":"LAT assembly into a two-dimensional protein condensate is a prominent feature of antigen discrimination by T cells. Here, we use single-molecule imaging techniques to resolve the spatial position and temporal duration of each pMHC:TCR molecular binding event while simultaneously monitoring LAT condensation at the membrane. An individual binding event is sufficient to trigger a LAT condensate, which is self-limiting, and neither its size nor lifetime is correlated with the duration of the originating pMHC:TCR binding event. Only the probability of the LAT condensate forming is related to the pMHC:TCR binding dwell time. LAT condenses abruptly, but after an extended delay from the originating binding event. A LAT mutation that facilitates phosphorylation at the PLC-γ1 recruitment site shortens the delay time to LAT condensation and alters T cell antigen specificity. These results identify a function for the LAT protein condensation phase transition in setting antigen discrimination thresholds in T cells.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-06-03T13:21:34.73Z","creation":"2025-04-05T19:07:15.197Z"},"accession":"S-EPMC9718779","cross_references":{"pubmed":["36460640"],"doi":["10.1038/s41467-022-35093-9"]}}