{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nie CH"],"funding":["Earmarked Fund for China Agriculture Research System","Ministry of Education","CAS","National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["nwac152"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9718792"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(11)"],"pubmed_abstract":["Intermuscular bones (IBs) are mineralized spicules, present in the myosepta of many, but not all, teleost species. IBs are often small and sharp, and they consequently limit how the fish can be processed; the IBs may cause injury or trauma if lodged in consumers' throats or mouths, and therefore affect the appeal of the fish to many consumers. The development of IBs in teleosts is still not fully understood and the molecular basis of IB development remains to be established. Here, the characteristics of IB tissue are evaluated based on single-cell transcriptomics in wild-type zebrafish. The analysis defined 18 distinct cell types. Differentiation trajectories showed that IBs are derived from tendons and that a core tendon-osteoblast cell lineage is related to IB formation. In particular, t"],"journal":["National science review"],"pubmed_title":["Single-cell transcriptomes and <i>runx2b<sup>-/-</sup></i> mutants reveal the genetic signatures of intermuscular bone formation in zebrafish."],"pmcid":["PMC9718792"],"funding_grant_id":["CARS-45-01","2021ZL08","41825013","U21A20263","2018YFD0900102","ZDBS-LY-DQC004","31872559"],"pubmed_authors":["Wang WM","Lin Q","Zhou JJ","Huysseune A","Hilsdorf AWS","Gao ZX","Wan SM","Wu YM","Witten PE","Nie CH","Chen YL"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomes and <i>runx2b<sup>-/-</sup></i> mutants reveal the genetic signatures of intermuscular bone formation in zebrafish.","description":"Intermuscular bones (IBs) are mineralized spicules, present in the myosepta of many, but not all, teleost species. IBs are often small and sharp, and they consequently limit how the fish can be processed; the IBs may cause injury or trauma if lodged in consumers' throats or mouths, and therefore affect the appeal of the fish to many consumers. The development of IBs in teleosts is still not fully understood and the molecular basis of IB development remains to be established. Here, the characteristics of IB tissue are evaluated based on single-cell transcriptomics in wild-type zebrafish. The analysis defined 18 distinct cell types. Differentiation trajectories showed that IBs are derived from tendons and that a core tendon-osteoblast cell lineage is related to IB formation. In particular, t","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-04-08T11:25:39.125Z","creation":"2024-12-04T12:29:53.051Z"},"accession":"S-EPMC9718792","cross_references":{"pubmed":["36478733"],"doi":["10.1093/nsr/nwac152"]}}