{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Durham EL"],"funding":["NIDCR NIH HHS","NCATS NIH HHS","NIGMS NIH HHS"],"pagination":["121158"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9719041"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["311(Pt A)"],"pubmed_abstract":["<h4>Aims</h4>Evidence suggests alterations of thyroid hormone levels can disrupt normal bone development. Most data suggest the major targets of thyroid hormones to be the Htra1/Igf1 pathway. Recent discovery by our group suggests involvement of targets WNT pathway, specifically overexpression of antagonist Sfrp4 in the presence of exogenous thyroid hormone.<h4>Main methods</h4>Here we aimed to model these interactions in vitro using primary and isotype cell lines to determine if thyroid hormone drives increased Sfrp4 expression in cells relevant to craniofacial development. Transcriptional profiling, bioinformatics interrogation, protein and function analyses were used.<h4>Key findings</h4>Affymetrix transcriptional profiling found Sfrp4 overexpression in primary cranial suture derived ce"],"journal":["Life sciences"],"pubmed_title":["Sfrp4 expression in thyroxine treated calvarial cells."],"pmcid":["PMC9719041"],"funding_grant_id":["UL1 TR000062","F31 DE026684","P30 GM103331","P30 GM103342","P20 GM103499","T32 DE017551","R03 DE023350"],"pubmed_authors":["Cray JJ","Black L","Grey ZJ","Lee BS","Durham EL","Howie RN","Barth JL"],"additional_accession":[]},"is_claimable":false,"name":"Sfrp4 expression in thyroxine treated calvarial cells.","description":"<h4>Aims</h4>Evidence suggests alterations of thyroid hormone levels can disrupt normal bone development. Most data suggest the major targets of thyroid hormones to be the Htra1/Igf1 pathway. Recent discovery by our group suggests involvement of targets WNT pathway, specifically overexpression of antagonist Sfrp4 in the presence of exogenous thyroid hormone.<h4>Main methods</h4>Here we aimed to model these interactions in vitro using primary and isotype cell lines to determine if thyroid hormone drives increased Sfrp4 expression in cells relevant to craniofacial development. Transcriptional profiling, bioinformatics interrogation, protein and function analyses were used.<h4>Key findings</h4>Affymetrix transcriptional profiling found Sfrp4 overexpression in primary cranial suture derived ce","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-05-09T03:13:11.625Z","creation":"2025-04-04T03:00:11.614Z"},"accession":"S-EPMC9719041","cross_references":{"pubmed":["36370870"],"doi":["10.1016/j.lfs.2022.121158"]}}