{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Niazi A"],"pubmed_abstract":["A homemade spectral shift fluorescence microscope (SSFM) is coupled with a spectrometer to record the spectral images of specimens based on the emission wavelength. Here a reliable diagnosis of neoplasia is achieved according to the spectral fluorescence properties of ex-vivo skin tissues after rhodamine6G (Rd6G) staining. It is shown that certain spectral shifts occur for nonmelanoma/melanoma lesions against normal/benign nevus, leading to spectral micrographs. In fact, there is a strong correlation between the emission wavelength and the sort of skin lesions, mainly due to the Rd6G interaction with the mitochondria of cancerous cells. The normal tissues generally enjoy a significant red shift regarding the laser line (37 nm). Conversely, plenty of fluorophores are conjugated to unhealthy"],"journal":["Scientific reports"],"pagination":["20927"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9719548"],"repository":["biostudies-literature"],"pubmed_title":["Discrimination of normal and cancerous human skin tissues based on laser-induced spectral shift fluorescence microscopy."],"pmcid":["PMC9719548"],"pubmed_authors":["Khodabakhshi Z","Basam MA","Bavali A","Shirafkan Dizaj L","Ehtesham A","Jafargholi A","Hassanzadeh T","Kamyab Hesari K","Sohrabizadeh Z","Moafi A","Niazi A","Amiri R","Atyabi F","Parvin P","Heidari O","Aghaei M"],"additional_accession":[]},"is_claimable":false,"name":"Discrimination of normal and cancerous human skin tissues based on laser-induced spectral shift fluorescence microscopy.","description":"A homemade spectral shift fluorescence microscope (SSFM) is coupled with a spectrometer to record the spectral images of specimens based on the emission wavelength. Here a reliable diagnosis of neoplasia is achieved according to the spectral fluorescence properties of ex-vivo skin tissues after rhodamine6G (Rd6G) staining. It is shown that certain spectral shifts occur for nonmelanoma/melanoma lesions against normal/benign nevus, leading to spectral micrographs. In fact, there is a strong correlation between the emission wavelength and the sort of skin lesions, mainly due to the Rd6G interaction with the mitochondria of cancerous cells. The normal tissues generally enjoy a significant red shift regarding the laser line (37 nm). Conversely, plenty of fluorophores are conjugated to unhealthy","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-04T13:46:31.847Z","creation":"2025-04-04T13:46:31.847Z"},"accession":"S-EPMC9719548","cross_references":{"pubmed":["36463297"],"doi":["10.1038/s41598-022-25055-y"]}}