biostudies-literatureUnknown12(1)Niazi AA homemade spectral shift fluorescence microscope (SSFM) is coupled with a spectrometer to record the spectral images of specimens based on the emission wavelength. Here a reliable diagnosis of neoplasia is achieved according to the spectral fluorescence properties of ex-vivo skin tissues after rhodamine6G (Rd6G) staining. It is shown that certain spectral shifts occur for nonmelanoma/melanoma lesions against normal/benign nevus, leading to spectral micrographs. In fact, there is a strong correlation between the emission wavelength and the sort of skin lesions, mainly due to the Rd6G interaction with the mitochondria of cancerous cells. The normal tissues generally enjoy a significant red shift regarding the laser line (37 nm). Conversely, plenty of fluorophores are conjugated to unhealthyScientific reports20927https://www.ebi.ac.uk/biostudies/studies/S-EPMC9719548biostudies-literatureDiscrimination of normal and cancerous human skin tissues based on laser-induced spectral shift fluorescence microscopy.PMC9719548Khodabakhshi ZBasam MABavali AShirafkan Dizaj LEhtesham AJafargholi AHassanzadeh TKamyab Hesari KSohrabizadeh ZMoafi ANiazi AAmiri RAtyabi FParvin PHeidari OAghaei MfalseDiscrimination of normal and cancerous human skin tissues based on laser-induced spectral shift fluorescence microscopy.A homemade spectral shift fluorescence microscope (SSFM) is coupled with a spectrometer to record the spectral images of specimens based on the emission wavelength. Here a reliable diagnosis of neoplasia is achieved according to the spectral fluorescence properties of ex-vivo skin tissues after rhodamine6G (Rd6G) staining. It is shown that certain spectral shifts occur for nonmelanoma/melanoma lesions against normal/benign nevus, leading to spectral micrographs. In fact, there is a strong correlation between the emission wavelength and the sort of skin lesions, mainly due to the Rd6G interaction with the mitochondria of cancerous cells. The normal tissues generally enjoy a significant red shift regarding the laser line (37 nm). Conversely, plenty of fluorophores are conjugated to unhealthy2022-01-01T00:00:00Z2022 Dec2025-04-04T13:46:31.847Z2025-04-04T13:46:31.847ZS-EPMC97195483646329710.1038/s41598-022-25055-y