{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Zhou Y"],"pubmed_abstract":["<b>Objective:</b> Site<b>-</b> and structure-specific quantitative N-glycoproteomics study of differential cell-surface N-glycosylation of ovarian cancer SKOV3 cells with the non-cancerous ovarian epithelial IOSE80 cells as the control. <b>Methods:</b> C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets. <b>Results:</b> With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure informati"],"journal":["Frontiers in chemistry"],"pagination":["1010642"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9723240"],"repository":["biostudies-literature"],"pubmed_title":["Comparative glycoproteomics study on the surface of SKOV3 <i>versus</i> IOSE80 cell lines."],"pmcid":["PMC9723240"],"pubmed_authors":["Wu L","Zhou Y","Cai X","Lin N"],"additional_accession":[]},"is_claimable":false,"name":"Comparative glycoproteomics study on the surface of SKOV3 <i>versus</i> IOSE80 cell lines.","description":"<b>Objective:</b> Site<b>-</b> and structure-specific quantitative N-glycoproteomics study of differential cell-surface N-glycosylation of ovarian cancer SKOV3 cells with the non-cancerous ovarian epithelial IOSE80 cells as the control. <b>Methods:</b> C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets. <b>Results:</b> With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure informati","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T14:32:07.685Z","creation":"2024-12-04T12:30:02.97Z"},"accession":"S-EPMC9723240","cross_references":{"pubmed":["36482940"],"doi":["10.3389/fchem.2022.1010642"]}}