{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jing X"],"funding":["National Natural Science Foundation of China","National Natural Science Foundation of China (National Science Foundation of China)"],"pagination":["106"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9723661"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(1)"],"pubmed_abstract":["Due to the challenges in detecting in situ activity and cultivating the not-yet-cultured, functional assessment and mining of living microbes from nature has typically followed a 'culture-first' paradigm. Here, employing phosphate-solubilizing microbes (PSM) as model, we introduce a 'screen-first' strategy that is underpinned by a precisely one-cell-resolution, complete workflow of single-cell Raman-activated Sorting and Cultivation (scRACS-Culture). Directly from domestic sewage, individual cells were screened for in-situ organic-phosphate-solubilizing activity via D<sub>2</sub>O intake rate, sorted by the function via Raman-activated Gravity-driven Encapsulation (RAGE), and then cultivated from precisely one cell. By scRACS-Culture, pure cultures of strong organic PSM including Comamonas"],"journal":["ISME communications"],"pubmed_title":["Single-cell Raman-activated sorting and cultivation (scRACS-Culture) for assessing and mining in situ phosphate-solubilizing microbes from nature."],"pmcid":["PMC9723661"],"funding_grant_id":["32030003"],"pubmed_authors":["Zhang J","Cui L","Wang C","Ren Y","Gong Y","Meng Y","Xu J","Pan H","Jing X","Li Y","Ma B","Diao Z","Qu L","Xu T","Ji Y","Mu R"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell Raman-activated sorting and cultivation (scRACS-Culture) for assessing and mining in situ phosphate-solubilizing microbes from nature.","description":"Due to the challenges in detecting in situ activity and cultivating the not-yet-cultured, functional assessment and mining of living microbes from nature has typically followed a 'culture-first' paradigm. Here, employing phosphate-solubilizing microbes (PSM) as model, we introduce a 'screen-first' strategy that is underpinned by a precisely one-cell-resolution, complete workflow of single-cell Raman-activated Sorting and Cultivation (scRACS-Culture). Directly from domestic sewage, individual cells were screened for in-situ organic-phosphate-solubilizing activity via D<sub>2</sub>O intake rate, sorted by the function via Raman-activated Gravity-driven Encapsulation (RAGE), and then cultivated from precisely one cell. By scRACS-Culture, pure cultures of strong organic PSM including Comamonas","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-05-28T12:56:20.861Z","creation":"2025-04-04T08:34:47.504Z"},"accession":"S-EPMC9723661","cross_references":{"pubmed":["37938284"],"doi":["10.1038/s43705-022-00188-3"]}}