{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Calcines-Cruz C"],"funding":["Direcci?n General de Asuntos del Personal Acad?mico, Universidad Nacional Aut?noma de M?xico","University of Texas at Austin","Welch Foundation","Consejo Nacional de Ciencia y Tecnolog?a","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2752-2757"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9724498"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(7)"],"pubmed_abstract":["Designer virus-inspired proteins drive the manufacturing of more effective, safer gene-delivery systems and simpler models to study viral assembly. However, self-assembly of engineered viromimetic proteins on specific nucleic acid templates, a distinctive viral property, has proved difficult. Inspired by viral packaging signals, we harness the programmability of CRISPR-Cas12a to direct the nucleation and growth of a self-assembling synthetic polypeptide into virus-like particles (VLP) on specific DNA molecules. Positioning up to ten nuclease-dead Cas12a (dCas12a) proteins along a 48.5 kbp DNA template triggers particle growth and full DNA encapsidation at limiting polypeptide concentrations. Particle growth rate is further increased when dCas12a is dimerized with a polymerization silk-like"],"journal":["Nano letters"],"pubmed_title":["CRISPR-Guided Programmable Self-Assembly of Artificial Virus-Like Nucleocapsids."],"pmcid":["PMC9724498"],"funding_grant_id":["R01 GM124141","IA200119","GM124141","F-1016"],"pubmed_authors":["Calcines-Cruz C","Finkelstein IJ","Hernandez-Garcia A"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR-Guided Programmable Self-Assembly of Artificial Virus-Like Nucleocapsids.","description":"Designer virus-inspired proteins drive the manufacturing of more effective, safer gene-delivery systems and simpler models to study viral assembly. However, self-assembly of engineered viromimetic proteins on specific nucleic acid templates, a distinctive viral property, has proved difficult. Inspired by viral packaging signals, we harness the programmability of CRISPR-Cas12a to direct the nucleation and growth of a self-assembling synthetic polypeptide into virus-like particles (VLP) on specific DNA molecules. Positioning up to ten nuclease-dead Cas12a (dCas12a) proteins along a 48.5 kbp DNA template triggers particle growth and full DNA encapsidation at limiting polypeptide concentrations. Particle growth rate is further increased when dCas12a is dimerized with a polymerization silk-like","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-19T05:54:46.14Z","creation":"2025-04-19T05:54:46.14Z"},"accession":"S-EPMC9724498","cross_references":{"pubmed":["33729813"],"doi":["10.1021/acs.nanolett.0c04640"]}}