biostudies-literatureUnknown13Liu W<h4>Introduction</h4>With society development, the age at which women choose to have children has been gradually delayed. To improve the reduced fertility in women at advanced maternal age, we developed a combination containing natural extracts from clove, Sophora flower bud and Chinese yam with a mass ratio 15:6:10 and named it as DACHAO.<h4>Methods and results</h4>We then gavage DACHAO at a dose of 310 mg/kg BW to female mice at 10 month of age and investigated its effects on ovarian functions. Using MitoTracker probes, ROS, and JC-1 staining, we found that DACHAO treatment improved mitochondria functions in oocytes from aged mice. We also observed increased blastocyst formation when mature oocytes from control and DACHAO treated mice were for IVF and in vitro embryo culture. Cell countiFrontiers in endocrinology945690https://www.ebi.ac.uk/biostudies/studies/S-EPMC9724743biostudies-literatureA combination containing natural extracts of clove, Sophora flower bud, and yam improves fertility in aged female mice <i>via</i> multiple mechanisms.PMC9724743Li JChen JZhang JZhang HChen YWang XWu TLiu WfalseA combination containing natural extracts of clove, Sophora flower bud, and yam improves fertility in aged female mice <i>via</i> multiple mechanisms.<h4>Introduction</h4>With society development, the age at which women choose to have children has been gradually delayed. To improve the reduced fertility in women at advanced maternal age, we developed a combination containing natural extracts from clove, Sophora flower bud and Chinese yam with a mass ratio 15:6:10 and named it as DACHAO.<h4>Methods and results</h4>We then gavage DACHAO at a dose of 310 mg/kg BW to female mice at 10 month of age and investigated its effects on ovarian functions. Using MitoTracker probes, ROS, and JC-1 staining, we found that DACHAO treatment improved mitochondria functions in oocytes from aged mice. We also observed increased blastocyst formation when mature oocytes from control and DACHAO treated mice were for IVF and in vitro embryo culture. Cell counti2022-01-01T00:00:00Z20222025-04-22T08:37:16.562Z2025-04-05T22:35:42.658ZS-EPMC97247433648300010.3389/fendo.2022.945690