biostudies-literatureUnknown22(1)Hymel E<h4>Background</h4>Nonalcoholic fatty liver disease affects about 24% of the world's population and may progress to nonalcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC). While more common in those that are obese, NASH-HCC can develop in lean individuals. The mechanisms by which HCC develops and the role of epigenetic changes in the context of obesity and normal weight are not well understood.<h4>Methods</h4>In this study, we used previously generated mouse models of lean and obese HCC using a choline deficient/high trans-fat/fructose/cholesterol diet and a choline supplemented/high trans-fat/fructose/cholesterol diet, respectively, to evaluate methylation differences in HCC progression in lean versus obese mice. Differentially methylated regions were determineBMC cancer1276https://www.ebi.ac.uk/biostudies/studies/S-EPMC9727966biostudies-literatureDifferential methylation patterns in lean and obese non-alcoholic steatohepatitis-associated hepatocellular carcinoma.PMC9727966Hymel EFarazi PAFisher KWfalseDifferential methylation patterns in lean and obese non-alcoholic steatohepatitis-associated hepatocellular carcinoma.<h4>Background</h4>Nonalcoholic fatty liver disease affects about 24% of the world's population and may progress to nonalcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC). While more common in those that are obese, NASH-HCC can develop in lean individuals. The mechanisms by which HCC develops and the role of epigenetic changes in the context of obesity and normal weight are not well understood.<h4>Methods</h4>In this study, we used previously generated mouse models of lean and obese HCC using a choline deficient/high trans-fat/fructose/cholesterol diet and a choline supplemented/high trans-fat/fructose/cholesterol diet, respectively, to evaluate methylation differences in HCC progression in lean versus obese mice. Differentially methylated regions were determine2022-01-01T00:00:00Z2022 Dec2025-04-19T06:36:21.891Z2025-04-19T06:36:21.891ZS-EPMC97279663647418310.1186/s12885-022-10389-7