{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["30(7)"],"submitter":["Zhou X"],"pubmed_abstract":["Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed <i>Ochrobactrum tritici</i> 5bvl1 ChrB in <i>Escherichia coli</i> BL21(DE3) cells. <i>E. coli</i> cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible"],"journal":["Journal of microbiology and biotechnology"],"pagination":["996-1004"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728187"],"repository":["biostudies-literature"],"pubmed_title":["Removal of Chromium (VI) by <i>Escherichia coli</i> Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study."],"pmcid":["PMC9728187"],"pubmed_authors":["Ren X","Yang J","Li J","Jiang F","Tan G","Zhou X","Yang F","Liang F","Lyu J","Cheng R","Wang W","Zhang C","Fan B","Zhou H"],"additional_accession":[]},"is_claimable":false,"name":"Removal of Chromium (VI) by <i>Escherichia coli</i> Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study.","description":"Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed <i>Ochrobactrum tritici</i> 5bvl1 ChrB in <i>Escherichia coli</i> BL21(DE3) cells. <i>E. coli</i> cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jul","modification":"2026-05-27T12:03:08.824Z","creation":"2024-11-08T20:11:21.35Z"},"accession":"S-EPMC9728187","cross_references":{"pubmed":["32238765"],"doi":["10.4014/jmb.1912.12030"]}}