biostudies-literatureUnknown30(7)Zhou XVarious genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed <i>Ochrobactrum tritici</i> 5bvl1 ChrB in <i>Escherichia coli</i> BL21(DE3) cells. <i>E. coli</i> cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptibleJournal of microbiology and biotechnology996-1004https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728187biostudies-literatureRemoval of Chromium (VI) by <i>Escherichia coli</i> Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study.PMC9728187Ren XYang JLi JJiang FTan GZhou XYang FLiang FLyu JCheng RWang WZhang CFan BZhou HfalseRemoval of Chromium (VI) by <i>Escherichia coli</i> Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study.Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed <i>Ochrobactrum tritici</i> 5bvl1 ChrB in <i>Escherichia coli</i> BL21(DE3) cells. <i>E. coli</i> cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible2020-01-01T00:00:00Z2020 Jul2026-05-27T12:03:08.824Z2024-11-08T20:11:21.35ZS-EPMC97281873223876510.4014/jmb.1912.12030