{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["30(3)"],"submitter":["Nguyen TTH"],"pubmed_abstract":["Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-L-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-L-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-L-rhamnoside, was assayed in various cancer cells."],"journal":["Journal of microbiology and biotechnology"],"pagination":["398-403"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728250"],"repository":["biostudies-literature"],"pubmed_title":["Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli."],"pmcid":["PMC9728250"],"pubmed_authors":["Nguyen TTH","Liou K","Sin HJ","Sohng JK","Pandey RP","Jung HJ"],"additional_accession":[]},"is_claimable":false,"name":"Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli.","description":"Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-L-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-L-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-L-rhamnoside, was assayed in various cancer cells.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2025-04-04T08:56:02.14Z","creation":"2025-04-04T08:56:02.14Z"},"accession":"S-EPMC9728250","cross_references":{"pubmed":["31893599"],"doi":["10.4014/jmb.1911.11047"]}}