biostudies-literatureUnknown30(6)Khan MIIn the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5- trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequJournal of microbiology and biotechnology839-847https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728379biostudies-literatureA <i>Sporolactobacillus</i>-, <i>Clostridium</i>-, and <i>Paenibacillus</i>- Dominant Microbial Consortium Improved Anaerobic RDX Detoxification by Starch Addition.PMC9728379Kim SBashir SPark JKhan MICheema SAYoo KfalseA <i>Sporolactobacillus</i>-, <i>Clostridium</i>-, and <i>Paenibacillus</i>- Dominant Microbial Consortium Improved Anaerobic RDX Detoxification by Starch Addition.In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5- trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequ2020-01-01T00:00:00Z2020 Jun2026-05-27T18:42:23.266Z2025-06-01T01:56:16.97ZS-EPMC97283793216069910.4014/jmb.1910.10034