{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["30(6)"],"submitter":["Xi Y"],"pubmed_abstract":["Bicarbonate has been considered as a better approach for supplying CO<sub>2</sub> to microalgae cells microenvironments than gas bubbling owing t°Cost-effectiveness and easy operation. However, the β-carotene production was too low in <i>Dunaliella salina</i> cultivated with bicarbonate in previous studies. Also, the difference in photosynthetic efficiency between these tw°Carbon sources (bicarbonate and CO<sub>2</sub>) has seldom been discussed. In this study, the culture conditions, including NaHCO<sub>3</sub>, Ca<sup>2+</sup>, Mg<sup>2+</sup> and microelement concentrations, were optimized when bicarbonate was used as carbon source. Under optimized condition, a maximum biomass concentration of 0.71 g/l and corresponding β-carotene content of 4.76% were obtained, with β-carotene yield of 32.0 mg/l, much higher than previous studies with NaHCO<sub>3</sub>. Finally, these optimized conditions with bicarbonate were compared with CO<sub>2</sub> bubbling by online monitoring. There was a notable difference in <i>F<sub>v</sub>/F<sub>m</sub></i> value between cultivations with bicarbonate and CO<sub>2</sub>, but there was no difference in the <i>F<sub>v</sub>/F<sub>m</sub></i> periodic changing patterns. This indicates that the high concentration of NaHCO<sub>3</sub> used in this study served as a stress factor for β-carotene accumulation, although high productivity of biomass was still obtained."],"journal":["Journal of microbiology and biotechnology"],"pagination":["868-877"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728381"],"repository":["biostudies-literature"],"pubmed_title":["β-Carotene Production from <i>Dunaliella salina</i> Cultivated with Bicarbonate as Carbon Source."],"pmcid":["PMC9728381"],"pubmed_authors":["Xi Y","Xue S","Wang J","Chi Z"],"additional_accession":[]},"is_claimable":false,"name":"β-Carotene Production from <i>Dunaliella salina</i> Cultivated with Bicarbonate as Carbon Source.","description":"Bicarbonate has been considered as a better approach for supplying CO<sub>2</sub> to microalgae cells microenvironments than gas bubbling owing t°Cost-effectiveness and easy operation. However, the β-carotene production was too low in <i>Dunaliella salina</i> cultivated with bicarbonate in previous studies. Also, the difference in photosynthetic efficiency between these tw°Carbon sources (bicarbonate and CO<sub>2</sub>) has seldom been discussed. In this study, the culture conditions, including NaHCO<sub>3</sub>, Ca<sup>2+</sup>, Mg<sup>2+</sup> and microelement concentrations, were optimized when bicarbonate was used as carbon source. Under optimized condition, a maximum biomass concentration of 0.71 g/l and corresponding β-carotene content of 4.76% were obtained, with β-carotene yield of 32.0 mg/l, much higher than previous studies with NaHCO<sub>3</sub>. Finally, these optimized conditions with bicarbonate were compared with CO<sub>2</sub> bubbling by online monitoring. There was a notable difference in <i>F<sub>v</sub>/F<sub>m</sub></i> value between cultivations with bicarbonate and CO<sub>2</sub>, but there was no difference in the <i>F<sub>v</sub>/F<sub>m</sub></i> periodic changing patterns. This indicates that the high concentration of NaHCO<sub>3</sub> used in this study served as a stress factor for β-carotene accumulation, although high productivity of biomass was still obtained.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2025-05-29T16:30:11.536Z","creation":"2025-05-29T16:30:11.536Z"},"accession":"S-EPMC9728381","cross_references":{"pubmed":["32238762"],"doi":["10.4014/jmb.1910.10035"]}}