biostudies-literatureUnknown19(1)Prinz FAustrian Science Fund FWFThe microRNA-200 family has wide-ranging regulatory functions in cancer development and progression. Above all, it is strongly associated with the epithelial-to-mesenchymal transition (EMT), a process during which cells change their epithelial to a mesenchymal phenotype and acquire invasive characteristics. More recently, miR-200 family members have also been reported to impact the immune evasion of cancer cells by regulating the expression of immunoinhibitory immune checkpoints (ICs) like PD-L1. Therefore, we aimed to comprehensively characterize this miR-200 family as a regulatory interface between EMT and immune evasion mechanisms in biliary tract cancer. Initial correlation analyses and transient overexpression experiments using miRNA mimics suggested miR-200c-3p as a putative regulatoRNA biology1305-1315https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728468biostudies-literatureMicroRNA mimics can distort physiological microRNA effects on immune checkpoints by triggering an antiviral interferon response.PMC9728468Reicher AMayr CRinner BBalihodzic APichler MKiesslich TRiedl JGerger APrinz FBarth DAJonas KKargl JKlec CfalseMicroRNA mimics can distort physiological microRNA effects on immune checkpoints by triggering an antiviral interferon response.The microRNA-200 family has wide-ranging regulatory functions in cancer development and progression. Above all, it is strongly associated with the epithelial-to-mesenchymal transition (EMT), a process during which cells change their epithelial to a mesenchymal phenotype and acquire invasive characteristics. More recently, miR-200 family members have also been reported to impact the immune evasion of cancer cells by regulating the expression of immunoinhibitory immune checkpoints (ICs) like PD-L1. Therefore, we aimed to comprehensively characterize this miR-200 family as a regulatory interface between EMT and immune evasion mechanisms in biliary tract cancer. Initial correlation analyses and transient overexpression experiments using miRNA mimics suggested miR-200c-3p as a putative regulato2022-01-01T00:00:00Z2022 Jan2025-04-22T11:22:44.012Z2025-04-05T23:54:22.241ZS-EPMC97284683646956410.1080/15476286.2022.2152978