{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(12)"],"submitter":["Kolibachuk D"],"funding":["Rhode Island College Faculty Scholarship and Development Committee"],"pubmed_abstract":["While plastics have revolutionized our world, plastic waste has serious environmental and economic impacts. Polyhydroxyalkanoic acid (PHA) is a bacterial carbon and energy reserve shown to be both biodegradable and biocompatible and could potentially replace conventional plastics. However, cost-effective mass production remains elusive. Bacteria often accumulate PHA as cytoplasmic granules. PHA synthase creates the PHA polymer from acetoacyl-CoA monomers, while phasins are small multifunctional proteins that are found in abundance on the granule surface. The PHA synthase gene from a novel marine isolate, Vibrio B-18 (or B-18), was placed in the presence or absence of an upstream phasin gene in a runaway replication plasmid using polymerase chain reaction (PCR) technology. Plasmid gene expr"],"journal":["PloS one"],"pagination":["e0275597"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9728866"],"repository":["biostudies-literature"],"pubmed_title":["Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation."],"pmcid":["PMC9728866"],"pubmed_authors":["Ryder BJ","Woolsey A","Duquenoy M","Thakkar K","Nieves A","Pacheco Nunez M","Lopes MK","Valente NR","Kolibachuk D","Lyons ER"],"additional_accession":[]},"is_claimable":false,"name":"Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation.","description":"While plastics have revolutionized our world, plastic waste has serious environmental and economic impacts. Polyhydroxyalkanoic acid (PHA) is a bacterial carbon and energy reserve shown to be both biodegradable and biocompatible and could potentially replace conventional plastics. However, cost-effective mass production remains elusive. Bacteria often accumulate PHA as cytoplasmic granules. PHA synthase creates the PHA polymer from acetoacyl-CoA monomers, while phasins are small multifunctional proteins that are found in abundance on the granule surface. The PHA synthase gene from a novel marine isolate, Vibrio B-18 (or B-18), was placed in the presence or absence of an upstream phasin gene in a runaway replication plasmid using polymerase chain reaction (PCR) technology. Plasmid gene expr","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-08T14:30:37.696Z","creation":"2024-11-13T21:52:25.914Z"},"accession":"S-EPMC9728866","cross_references":{"pubmed":["36477445"],"doi":["10.1371/journal.pone.0275597"]}}