{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Barmukh R"],"funding":["Department of Science and Technology, Government of India","Council of Scientific and Industrial Research (CSIR), Government of India","Department of Science & Technology (DST), Government of India","Department of Biotechnology, Government of India","Bill and Melinda Gates Foundation"],"pagination":["7255-7272"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9730794"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["73(22)"],"pubmed_abstract":["'QTL-hotspot' is a genomic region on linkage group 04 (CaLG04) in chickpea (Cicer arietinum) that harbours major-effect quantitative trait loci (QTLs) for multiple drought-adaptive traits, and it therefore represents a promising target for improving drought adaptation. To investigate the mechanisms underpinning the positive effects of 'QTL-hotspot' on seed yield under drought, we introgressed this region from the ICC 4958 genotype into five elite chickpea cultivars. The resulting introgression lines (ILs) and their parents were evaluated in multi-location field trials and semi-controlled conditions. The results showed that the 'QTL-hotspot' region improved seed yield under rainfed conditions by increasing seed weight, reducing the time to flowering, regulating traits related to canopy growth and early vigour, and enhancing transpiration efficiency. Whole-genome sequencing data analysis of the ILs and parents revealed four genes underlying the 'QTL-hotspot' region associated with drought adaptation. We validated diagnostic KASP markers closely linked to these genes using the ILs and their parents for future deployment in chickpea breeding programs. The CaTIFY4b-H2 haplotype of a potential candidate gene CaTIFY4b was identified as the superior haplotype for 100-seed weight. The candidate genes and superior haplotypes identified in this study have the potential to serve as direct targets for genetic manipulation and selection for chickpea improvement."],"journal":["Journal of experimental botany"],"pubmed_title":["Characterization of 'QTL-hotspot' introgression lines reveals physiological mechanisms and candidate genes associated with drought adaptation in chickpea."],"pmcid":["PMC9730794"],"funding_grant_id":["SB/S9/Z-13/2019"],"pubmed_authors":["Bajaj P","Sreeman SM","Barmukh R","Smith MR","Rao Sagurthi S","Dixit GP","Yasin M","Siddique KHM","Tripathi S","Bharadwaj C","Kholova J","Chitikineni A","Vijayakumar AG","Rathore A","Roorkiwal M","Varshney RK"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of 'QTL-hotspot' introgression lines reveals physiological mechanisms and candidate genes associated with drought adaptation in chickpea.","description":"'QTL-hotspot' is a genomic region on linkage group 04 (CaLG04) in chickpea (Cicer arietinum) that harbours major-effect quantitative trait loci (QTLs) for multiple drought-adaptive traits, and it therefore represents a promising target for improving drought adaptation. To investigate the mechanisms underpinning the positive effects of 'QTL-hotspot' on seed yield under drought, we introgressed this region from the ICC 4958 genotype into five elite chickpea cultivars. The resulting introgression lines (ILs) and their parents were evaluated in multi-location field trials and semi-controlled conditions. The results showed that the 'QTL-hotspot' region improved seed yield under rainfed conditions by increasing seed weight, reducing the time to flowering, regulating traits related to canopy growth and early vigour, and enhancing transpiration efficiency. Whole-genome sequencing data analysis of the ILs and parents revealed four genes underlying the 'QTL-hotspot' region associated with drought adaptation. We validated diagnostic KASP markers closely linked to these genes using the ILs and their parents for future deployment in chickpea breeding programs. The CaTIFY4b-H2 haplotype of a potential candidate gene CaTIFY4b was identified as the superior haplotype for 100-seed weight. The candidate genes and superior haplotypes identified in this study have the potential to serve as direct targets for genetic manipulation and selection for chickpea improvement.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2024-10-18T13:10:41.813Z","creation":"2024-10-18T13:10:41.813Z"},"accession":"S-EPMC9730794","cross_references":{"pubmed":["36006832"],"doi":["10.1093/jxb/erac348"]}}