biostudies-literatureStasi MH2020 Marie Sklodowska-Curie ActionsEuropean Research CouncilDeutsche ForschungsgemeinschaftTechnische Universit?t M?nchen21939-21947https://www.ebi.ac.uk/biostudies/studies/S-EPMC9732876biostudies-literatureUnknown144(48)Molecular machines, such as ATPases or motor proteins, couple the catalysis of a chemical reaction, most commonly hydrolysis of nucleotide triphosphates, to their conformational change. In essence, they continuously convert a chemical fuel to drive their motion. An outstanding goal of nanotechnology remains to synthesize a nanomachine with similar functions, precision, and speed. The field of DNA nanotechnology has given rise to the engineering precision required for such a device. Simultaneously, the field of systems chemistry developed fast chemical reaction cycles that convert fuel to change the function of molecules. In this work, we thus combined a chemical reaction cycle with the precision of DNA nanotechnology to yield kinetic control over the conformational state of a DNA hairpin. Journal of the American Chemical SocietyRegulating DNA-Hybridization Using a Chemically Fueled Reaction Cycle.PMC9732876724261765703EXC-2094 411722921852187EXC-2094 390783311Babl LDirscherl CFBoekhoven JDietz HStasi MWunnava SMonferrer ASchwille PBraun DfalseRegulating DNA-Hybridization Using a Chemically Fueled Reaction Cycle.Molecular machines, such as ATPases or motor proteins, couple the catalysis of a chemical reaction, most commonly hydrolysis of nucleotide triphosphates, to their conformational change. In essence, they continuously convert a chemical fuel to drive their motion. An outstanding goal of nanotechnology remains to synthesize a nanomachine with similar functions, precision, and speed. The field of DNA nanotechnology has given rise to the engineering precision required for such a device. Simultaneously, the field of systems chemistry developed fast chemical reaction cycles that convert fuel to change the function of molecules. In this work, we thus combined a chemical reaction cycle with the precision of DNA nanotechnology to yield kinetic control over the conformational state of a DNA hairpin. 2022-01-01T00:00:00Z2022 Dec2025-04-20T03:30:23.992Z2025-04-20T03:30:23.992ZS-EPMC97328763644285010.1021/jacs.2c08463