{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Nagao K"],"pubmed_abstract":["In the Japanese hagfish Eptatretus burgeri, 16 chromosomes (eliminated [E]-chromosomes) have been lost in somatic cells (2n = 36), which is equivalent to approx. 21% of the genomic DNA in germ cells (2n = 52). At least seven of the 12 eliminated repetitive DNA families isolated in eight hagfish species were selectively amplified in the germline genome of this species. One of them, EEEb1 (eliminated element of E. burgeri 1) is exclusively localized on all E-chromosomes. Herein, we identified four novel eliminated repetitive DNA families (named EEEb3-6) through PCR amplification and suppressive subtractive hybridization (SSH) combined with Southern-blot hybridization. EEEb3 was mosaic for 5S rDNA and SINE elements. EEEb4 was GC-rich repeats and has one pair of direct and inverted repeats, wh"],"journal":["Scientific reports"],"pagination":["21373"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9734144"],"repository":["biostudies-literature"],"pubmed_title":["Novel selectively amplified DNA sequences in the germline genome of the Japanese hagfish, Eptatretus burgeri."],"pmcid":["PMC9734144"],"pubmed_authors":["Chinone H","Yoshimoto J","Goto Y","Nagao K","Kubota S","Sasaki T","Matsuda M","Otsuzumi T"],"additional_accession":[]},"is_claimable":false,"name":"Novel selectively amplified DNA sequences in the germline genome of the Japanese hagfish, Eptatretus burgeri.","description":"In the Japanese hagfish Eptatretus burgeri, 16 chromosomes (eliminated [E]-chromosomes) have been lost in somatic cells (2n = 36), which is equivalent to approx. 21% of the genomic DNA in germ cells (2n = 52). At least seven of the 12 eliminated repetitive DNA families isolated in eight hagfish species were selectively amplified in the germline genome of this species. One of them, EEEb1 (eliminated element of E. burgeri 1) is exclusively localized on all E-chromosomes. Herein, we identified four novel eliminated repetitive DNA families (named EEEb3-6) through PCR amplification and suppressive subtractive hybridization (SSH) combined with Southern-blot hybridization. EEEb3 was mosaic for 5S rDNA and SINE elements. EEEb4 was GC-rich repeats and has one pair of direct and inverted repeats, wh","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-07-14T15:53:59.484Z","creation":"2025-04-04T23:36:54.594Z"},"accession":"S-EPMC9734144","cross_references":{"pubmed":["36494570"],"doi":["10.1038/s41598-022-26007-2"]}}