biostudies-literatureUnknown12(23)Mikheev IVA semi-automated diffusion-dialysis purification procedure is proposed for the preparation of uncontaminated graphene oxide (GO) aqueous dispersions. The purification process is integrated with analytical-signal processing to control the purification degree online by several channels: oxidation-reduction potential, conductivity, and absorbance. This approach reduces the amounts of reagents for chemical treatment during dialysis. The total transition metal (Mn and Ti) content was reduced to a sub-ppb level (assessed by slurry nebulization in inductively coupled plasma optical atomic emission spectroscopy). Purified aqueous GO samples possess good stability for about a year with a zeta-potential of ca. -40 mV and a lateral size of ca. sub-µm. Purified GO samples showed increased antioxidant properties (up to five times compared to initial samples according to chemiluminometry by superoxide-radical (O2-) generated in situ from xanthine and xanthine oxidase with the lucigenin probe) and significantly decreased peroxidase-like activity (assessed by the H2O2-L-012 system).Nanomaterials (Basel, Switzerland)4159https://www.ebi.ac.uk/biostudies/studies/S-EPMC9739863biostudies-literatureHigh-Throughput Preparation of Uncontaminated Graphene-Oxide Aqueous Dispersions with Antioxidant Properties by Semi-Automated Diffusion Dialysis.PMC9739863Byvsheva SMKottsov SYMikheev IVProskurnin MASozarukova MMProskurnina EVfalseHigh-Throughput Preparation of Uncontaminated Graphene-Oxide Aqueous Dispersions with Antioxidant Properties by Semi-Automated Diffusion Dialysis.A semi-automated diffusion-dialysis purification procedure is proposed for the preparation of uncontaminated graphene oxide (GO) aqueous dispersions. The purification process is integrated with analytical-signal processing to control the purification degree online by several channels: oxidation-reduction potential, conductivity, and absorbance. This approach reduces the amounts of reagents for chemical treatment during dialysis. The total transition metal (Mn and Ti) content was reduced to a sub-ppb level (assessed by slurry nebulization in inductively coupled plasma optical atomic emission spectroscopy). Purified aqueous GO samples possess good stability for about a year with a zeta-potential of ca. -40 mV and a lateral size of ca. sub-µm. Purified GO samples showed increased antioxidant properties (up to five times compared to initial samples according to chemiluminometry by superoxide-radical (O2-) generated in situ from xanthine and xanthine oxidase with the lucigenin probe) and significantly decreased peroxidase-like activity (assessed by the H2O2-L-012 system).2022-01-01T00:00:00Z2022 Nov2025-04-20T03:32:27.978Z2025-04-20T03:32:27.978ZS-EPMC97398633650078210.3390/nano12234159