biostudies-literatureRadulovic NSMinistry of Education, Science and Technological Development of the Republic of Serbia3340https://www.ebi.ac.uk/biostudies/studies/S-EPMC9739903biostudies-literatureUnknown11(23)Motivated by the ethnopharmacological use of <i>Pulicaria dysenterica</i>, in the present study, the antimicrobial potential of the extracted essential oil was investigated against a panel of eighteen microorganism strains. Additionally, anti-acetylcholinesterase and antispasmodic (isolated rat distal colon) activities, general acute toxicity (<i>Artemia salina</i> model), and immunomodulatory properties (cytotoxicity on isolated mouse macrophages) were studied. Detailed analyses of the essential oil led to the identification of 3-methoxycuminyl 2-methylbutanoate (a new natural product) and 3-methoxycuminyl 3-methylbutanoate (a rare natural product). The obtained esters and intermediates in the synthesis of the starting alcohol (3-methoxycuminol) were subjected to a battery of 1D- and 2D-NPlants (Basel, Switzerland)<i>Pulicaria dysenterica</i> (L.) Bernh.-Rightfully Earned Name? Identification and Biological Activity of New 3-Methoxycuminyl Esters from <i>P. dysenterica</i> Essential Oil.PMC9739903451-03-68/2022-14/200124 and 451-03-68/2022-14/200113Radulovic NSStojanovic NMBoylan FMladenovic MZRandjelovic PJVukicevic DRStojanovic-Radic ZZfalse<i>Pulicaria dysenterica</i> (L.) Bernh.-Rightfully Earned Name? Identification and Biological Activity of New 3-Methoxycuminyl Esters from <i>P. dysenterica</i> Essential Oil.Motivated by the ethnopharmacological use of <i>Pulicaria dysenterica</i>, in the present study, the antimicrobial potential of the extracted essential oil was investigated against a panel of eighteen microorganism strains. Additionally, anti-acetylcholinesterase and antispasmodic (isolated rat distal colon) activities, general acute toxicity (<i>Artemia salina</i> model), and immunomodulatory properties (cytotoxicity on isolated mouse macrophages) were studied. Detailed analyses of the essential oil led to the identification of 3-methoxycuminyl 2-methylbutanoate (a new natural product) and 3-methoxycuminyl 3-methylbutanoate (a rare natural product). The obtained esters and intermediates in the synthesis of the starting alcohol (3-methoxycuminol) were subjected to a battery of 1D- and 2D-N2022-01-01T00:00:00Z2022 Dec2025-04-18T19:19:49.809Z2024-11-19T15:15:15.49ZS-EPMC97399033650137910.3390/plants11233340