biostudies-literatureUnknown23(23)Domin RATPase inhibitory factor 1 is a myokine inhibiting the hydrolytic activity of mitochondrial adenosine triphosphate synthase and ecto-F1-ATPase on the surface of many cells. IF1 affects ATP metabolism in mitochondria and the extracellular space and upregulates glucose uptake in myocytes; these processes are essential in physical activity. It is unknown whether the IF1 serum concentration is associated with exercise capacity. This study explored the association between resting IF1 serum concentration and exercise capacity indices in healthy people. IF1 serum concentration was measured in samples collected at rest in 97 healthy amateur cyclists. Exercise capacity was assessed on a bike ergometer at the successive stages of the progressive cardiopulmonary exercise test (CPET). IF1 serum concenInternational journal of molecular sciences15303https://www.ebi.ac.uk/biostudies/studies/S-EPMC9741029biostudies-literatureATPase Inhibitory Factor 1-A Novel Marker of Cellular Fitness and Exercise Capacity?PMC9741029Zybek-Kocik AWrotkowska ERuchala MGuzik PZolynski MDomin RNizinski JPytka MZielinski JfalseATPase Inhibitory Factor 1-A Novel Marker of Cellular Fitness and Exercise Capacity?ATPase inhibitory factor 1 is a myokine inhibiting the hydrolytic activity of mitochondrial adenosine triphosphate synthase and ecto-F1-ATPase on the surface of many cells. IF1 affects ATP metabolism in mitochondria and the extracellular space and upregulates glucose uptake in myocytes; these processes are essential in physical activity. It is unknown whether the IF1 serum concentration is associated with exercise capacity. This study explored the association between resting IF1 serum concentration and exercise capacity indices in healthy people. IF1 serum concentration was measured in samples collected at rest in 97 healthy amateur cyclists. Exercise capacity was assessed on a bike ergometer at the successive stages of the progressive cardiopulmonary exercise test (CPET). IF1 serum concen2022-01-01T00:00:00Z2022 Dec2025-04-04T13:33:00.751Z2024-11-21T02:26:19.993ZS-EPMC97410293649963010.3390/ijms232315303