{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hatakeyama D"],"funding":["Waseda University"],"pagination":["e0279017"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9744327"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(12)"],"pubmed_abstract":["Quantitative real-time PCR (qPCR) is a powerful method for measuring nucleic acid levels and quantifying mRNA levels, even in single cells. In the present study, we compared the results of single-cell qPCR obtained by different quantification methods (relative and absolute) and different reverse transcription methods. In the experiments, we focused on the cerebral giant cell (CGC), a key neuron required for the acquisition of conditioned taste aversion in the pond snail Lymnaea stagnalis, and examined changes in the mRNA levels of 3 memory-related genes, cAMP-response element binding proteins (LymCREB1 and LymCREB2) and CREB-binding protein (LymCBP), during memory formation. The results obtained by relative quantification showed similar patterns for the 3 genes. For absolute quantification"],"journal":["PloS one"],"pubmed_title":["Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail."],"pmcid":["PMC9744327"],"funding_grant_id":["the Waseda University Grant for Special Research Projects 2018K-141"],"pubmed_authors":["Ito E","Kitahashi T","Hatakeyama D","Chikamoto N","Fujimoto K"],"additional_accession":[]},"is_claimable":false,"name":"Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail.","description":"Quantitative real-time PCR (qPCR) is a powerful method for measuring nucleic acid levels and quantifying mRNA levels, even in single cells. In the present study, we compared the results of single-cell qPCR obtained by different quantification methods (relative and absolute) and different reverse transcription methods. In the experiments, we focused on the cerebral giant cell (CGC), a key neuron required for the acquisition of conditioned taste aversion in the pond snail Lymnaea stagnalis, and examined changes in the mRNA levels of 3 memory-related genes, cAMP-response element binding proteins (LymCREB1 and LymCREB2) and CREB-binding protein (LymCBP), during memory formation. The results obtained by relative quantification showed similar patterns for the 3 genes. For absolute quantification","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-13T14:35:24.806Z","creation":"2024-10-16T03:57:10.094Z"},"accession":"S-EPMC9744327","cross_references":{"pubmed":["36508476"],"doi":["10.1371/journal.pone.0279017"]}}