{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Yang X"],"pubmed_abstract":["<i>Brucella abortus</i> (<i>B. abortus</i>) as an important infectious agent of bovine brucellosis cannot be ignored, especially in countries/regions dominated by animal husbandry. Thus, the development of an ultrasensitive and highly specific identification technique is an ideal strategy to control the transmission of bovine brucellosis. In this report, a novel detection protocol, which utilizes multiple cross displacement amplification (MCDA) combined with a gold nanoparticles-based lateral flow biosensor (AuNPs-LFB) targeting the <i>BruAb2_0168</i> gene was successfully devised and established for the identification of <i>B. abortus</i> (termed <i>B. abortus</i>-MCDA-LFB). Ten specific primers containing engineered C1-FAM (carboxyfluorescein) and D1-biotin primers were designed according to the MCDA reaction mechanism. These genomic DNA extracted from various bacterial strains and whole blood samples were used to optimize and evaluate the <i>B. abortus</i>-MCDA-LFB assay. As a result, the optimal reaction conditions for the <i>B. abortus</i>-MCDA-LFB assay were 66°C for 40 min. The limit of detection of the <i>B. abortus</i>-MCDA-LFB was 10 fg/μl (~3 copies/μl) for genomic DNA extracted from pure cultures of <i>B. abortus</i> isolate. Meanwhile, the <i>B. abortus</i>-MCDA-LFB assay accurately identified all tested <i>B. abortus</i> strains, and there was no cross-reaction with non-<i>B. abortus</i> pathogens. Moreover, the detection workflow of the <i>B. abortus</i>-MCDA-LFB assay for whole blood samples can be completed within 70 min, and the cost of a single test is approximately 5.0 USD. Taken together, the <i>B. abortus</i>-MCDA-LFB assay is a visual, fast, ultrasensitive, low-cost, easy-to-operate, and highly specific detection method, which can be used as a rapid identification tool for <i>B. abortus</i> infections."],"journal":["Frontiers in microbiology"],"pagination":["1071928"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9744775"],"repository":["biostudies-literature"],"pubmed_title":["Rapid, ultrasensitive, and highly specific identification of <i>Brucella abortus</i> utilizing multiple cross displacement amplification combined with a gold nanoparticles-based lateral flow biosensor."],"pmcid":["PMC9744775"],"pubmed_authors":["Yang X","Liu Y","Li S","Huang J","Wei X","Zeng X","Ying X","Wang Y","Tan Q"],"additional_accession":[]},"is_claimable":false,"name":"Rapid, ultrasensitive, and highly specific identification of <i>Brucella abortus</i> utilizing multiple cross displacement amplification combined with a gold nanoparticles-based lateral flow biosensor.","description":"<i>Brucella abortus</i> (<i>B. abortus</i>) as an important infectious agent of bovine brucellosis cannot be ignored, especially in countries/regions dominated by animal husbandry. Thus, the development of an ultrasensitive and highly specific identification technique is an ideal strategy to control the transmission of bovine brucellosis. In this report, a novel detection protocol, which utilizes multiple cross displacement amplification (MCDA) combined with a gold nanoparticles-based lateral flow biosensor (AuNPs-LFB) targeting the <i>BruAb2_0168</i> gene was successfully devised and established for the identification of <i>B. abortus</i> (termed <i>B. abortus</i>-MCDA-LFB). Ten specific primers containing engineered C1-FAM (carboxyfluorescein) and D1-biotin primers were designed according to the MCDA reaction mechanism. These genomic DNA extracted from various bacterial strains and whole blood samples were used to optimize and evaluate the <i>B. abortus</i>-MCDA-LFB assay. As a result, the optimal reaction conditions for the <i>B. abortus</i>-MCDA-LFB assay were 66°C for 40 min. The limit of detection of the <i>B. abortus</i>-MCDA-LFB was 10 fg/μl (~3 copies/μl) for genomic DNA extracted from pure cultures of <i>B. abortus</i> isolate. Meanwhile, the <i>B. abortus</i>-MCDA-LFB assay accurately identified all tested <i>B. abortus</i> strains, and there was no cross-reaction with non-<i>B. abortus</i> pathogens. Moreover, the detection workflow of the <i>B. abortus</i>-MCDA-LFB assay for whole blood samples can be completed within 70 min, and the cost of a single test is approximately 5.0 USD. Taken together, the <i>B. abortus</i>-MCDA-LFB assay is a visual, fast, ultrasensitive, low-cost, easy-to-operate, and highly specific detection method, which can be used as a rapid identification tool for <i>B. abortus</i> infections.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-04T12:01:14.868Z","creation":"2025-02-19T02:59:49.99Z"},"accession":"S-EPMC9744775","cross_references":{"pubmed":["36523830"],"doi":["10.3389/fmicb.2022.1071928"]}}