biostudies-literatureUnknownTrumpp MEuropean Research CouncilDeutsche ForschungsgemeinschaftMedical Research CouncilDiabetes UKSonnenfeld StiftungHerein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.Chemical communications (Cambridge, England)https://www.ebi.ac.uk/biostudies/studies/S-EPMC9745879biostudies-literatureEnzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy.PMC9745879SFB1444EP/X026833/1Starting Grant 101042046MR/N00275X/1GRK2318/TJ-Train A4MR/S025618/110104204617/0005681715884Starting Grant 715884Gonschior HBroichhagen JAst JTrumpp MBirol MKnaus PLehmann MOliveras AHodson DJfalseEnzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy.Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.2022-01-01T00:00:00Z2022 Nov2025-05-29T22:30:46.921Z2025-05-29T22:30:46.921ZS-EPMC97458793642702110.1039/d2cc04823j