biostudies-literatureHoltappels DVlaams agentschap Innoveren en Ondernemen VLAIO LAEuropean Union's Horizon H2020 Research and Innovation ProgrammeFonds voor Wetenschappelijk Onderzoek304https://www.ebi.ac.uk/biostudies/studies/S-EPMC9746216biostudies-literatureUnknown22(1)The growing interest in the therapeutic application of bacteriophages leads to a drastic increase in the number of sequenced genomes. Luckily, recent insights in phage taxonomy facilitate the classification of phages in a comprehensive and data-driven manner as recently proposed by the International Committee on Taxonomy of Viruses. In this research, we present the taxonomical classification of a novel, narrow host range Xanthomonas phage FoX4, isolated from a Brussels sprouts field in Belgium infested with Xanthomonas campestris pv. campestris. The phage has a limited ability to lyse a bacterial culture, yet adsorbs efficiently to its host. Based on its genome sequence and low similarity to previously described phages, the phage comprises the novel phage genus Foxquatrovirus.BMC microbiologyIsolation, characterization and genome analysis of an orphan phage FoX4 of the new Foxquatrovirus genus.PMC9746216N. 773567IWT.1509141S02520NHoltappels DLavigne RWagemans JFortuna KJVallino MfalseIsolation, characterization and genome analysis of an orphan phage FoX4 of the new Foxquatrovirus genus.The growing interest in the therapeutic application of bacteriophages leads to a drastic increase in the number of sequenced genomes. Luckily, recent insights in phage taxonomy facilitate the classification of phages in a comprehensive and data-driven manner as recently proposed by the International Committee on Taxonomy of Viruses. In this research, we present the taxonomical classification of a novel, narrow host range Xanthomonas phage FoX4, isolated from a Brussels sprouts field in Belgium infested with Xanthomonas campestris pv. campestris. The phage has a limited ability to lyse a bacterial culture, yet adsorbs efficiently to its host. Based on its genome sequence and low similarity to previously described phages, the phage comprises the novel phage genus Foxquatrovirus.2022-01-01T00:00:00Z2022 Dec2025-04-18T19:21:23.941Z2025-02-18T23:49:44.965ZS-EPMC97462163651399610.1186/s12866-022-02719-3