{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Schwister EM"],"funding":["U.S. Department of Agriculture","USDA | National Institute of Food and Agriculture","National Science Foundation"],"pagination":["e0124122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9746312"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(23)"],"pubmed_abstract":["Plant growth-promoting (PGP) bacteria are important to the development of sustainable agricultural systems. PGP microbes that fix atmospheric nitrogen (diazotrophs) could minimize the application of industrially derived fertilizers and function as a biofertilizer. The bacterium Gluconacetobacter diazotrophicus is a nitrogen-fixing PGP microbe originally discovered in association with sugarcane plants, where it functions as an endophyte. It also forms endophyte associations with a range of other agriculturally relevant crop plants. G. diazotrophicus requires microaerobic conditions for diazotrophic growth. We generated a transposon library for G. diazotrophicus and cultured the library under various growth conditions and culture medium compositions to measure fitness defects associated with individual transposon inserts (transposon insertion sequencing [Tn-seq]). Using this library, we probed more than 3,200 genes and ascertained the importance of various genes for diazotrophic growth of this microaerobic endophyte. We also identified a set of essential genes. <b>IMPORTANCE</b> Our results demonstrate a succinct set of genes involved in diazotrophic growth for G. diazotrophicus, with a lower degree of redundancy than what is found in other model diazotrophs. The results will serve as a valuable resource for those interested in biological nitrogen fixation and will establish a baseline data set for plant free growth, which could complement future studies related to the endophyte relationship."],"journal":["Applied and environmental microbiology"],"pubmed_title":["Gluconacetobacter diazotrophicus Gene Fitness during Diazotrophic Growth."],"pmcid":["PMC9746312"],"funding_grant_id":["2020-67019-31148","MIN-12-070","MIN-12-081","CBET-1437758"],"pubmed_authors":["Barney BM","Dietz BR","Olszewski NE","Schwister EM","Knutson CM"],"additional_accession":[]},"is_claimable":false,"name":"Gluconacetobacter diazotrophicus Gene Fitness during Diazotrophic Growth.","description":"Plant growth-promoting (PGP) bacteria are important to the development of sustainable agricultural systems. PGP microbes that fix atmospheric nitrogen (diazotrophs) could minimize the application of industrially derived fertilizers and function as a biofertilizer. The bacterium Gluconacetobacter diazotrophicus is a nitrogen-fixing PGP microbe originally discovered in association with sugarcane plants, where it functions as an endophyte. It also forms endophyte associations with a range of other agriculturally relevant crop plants. G. diazotrophicus requires microaerobic conditions for diazotrophic growth. We generated a transposon library for G. diazotrophicus and cultured the library under various growth conditions and culture medium compositions to measure fitness defects associated with individual transposon inserts (transposon insertion sequencing [Tn-seq]). Using this library, we probed more than 3,200 genes and ascertained the importance of various genes for diazotrophic growth of this microaerobic endophyte. We also identified a set of essential genes. <b>IMPORTANCE</b> Our results demonstrate a succinct set of genes involved in diazotrophic growth for G. diazotrophicus, with a lower degree of redundancy than what is found in other model diazotrophs. The results will serve as a valuable resource for those interested in biological nitrogen fixation and will establish a baseline data set for plant free growth, which could complement future studies related to the endophyte relationship.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-06-13T06:18:32.421Z","creation":"2025-02-19T02:37:59.887Z"},"accession":"S-EPMC9746312","cross_references":{"pubmed":["36374093"],"doi":["10.1128/aem.01241-22"]}}