biostudies-literatureCascella RFondazione Italiana di Ricerca per la Sclerosi Laterale AmiotroficaUniversità di Firenze-Fondazione Cassa di Risparmio di FirenzeUniversity of GenoaUniversity of FlorenceHitachiGrant Heavy Equipment72-88https://www.ebi.ac.uk/biostudies/studies/S-EPMC9746631biostudies-literatureUnknown55(1)Introduction: Several neurodegenerative conditions are associated with a common histopathology within neurons of the central nervous system, consisting of the deposition of cytoplasmic inclusions of TAR DNA-binding protein 43 (TDP-43). Such inclusions have variably been described as morphologically and molecularly ordered aggregates having amyloid properties, as filaments without the cross-β-structure and dye binding specific for amyloid, or as amorphous aggregates with no defined structure and fibrillar morphology.Aims and Methods: Here we have expressed human full-length TDP-43 in neuroblastoma x spinal cord 34 (NSC-34) cells to investigate the morphological, structural, and tinctorial properties of TDP-43 inclusions in situ. We have used last-generation amyloid diagAnnals of medicineAn in situ and in vitro investigation of cytoplasmic TDP-43 inclusions reveals the absence of a clear amyloid signature.PMC9746631HT7800 120 kV3404TDP-43-STRUCTTROTHERALSTDP43SLANatalello AAmi DCascella RChiti FBanchelli MStaderini TCecchi CTampellini DMatteini PAdibi HAbolghasem Ghadami SBigi ACortese KGagliani MCfalseAn in situ and in vitro investigation of cytoplasmic TDP-43 inclusions reveals the absence of a clear amyloid signature.Introduction: Several neurodegenerative conditions are associated with a common histopathology within neurons of the central nervous system, consisting of the deposition of cytoplasmic inclusions of TAR DNA-binding protein 43 (TDP-43). Such inclusions have variably been described as morphologically and molecularly ordered aggregates having amyloid properties, as filaments without the cross-β-structure and dye binding specific for amyloid, or as amorphous aggregates with no defined structure and fibrillar morphology.Aims and Methods: Here we have expressed human full-length TDP-43 in neuroblastoma x spinal cord 34 (NSC-34) cells to investigate the morphological, structural, and tinctorial properties of TDP-43 inclusions in situ. We have used last-generation amyloid diag2023-01-01T00:00:00Z2023 Dec2026-06-04T09:48:18.425Z2025-04-05T12:39:18.229ZS-EPMC97466313649526210.1080/07853890.2022.2148734