{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["45(6)"],"submitter":["Wu Y"],"funding":["Monash University"],"pubmed_abstract":["<h4>Background</h4>In prostate cancer, the tumour microenvironment (TME) represents an important regulator of disease progression and response to treatment. In the TME, cancer-associated fibroblasts (CAFs) play a key role in tumour progression, however the mechanisms underpinning fibroblast-cancer cell interactions are incompletely resolved. Here, we address this by applying cell type-specific labelling with amino acid precursors (CTAP) and mass spectrometry (MS)-based (phospho)proteomics to prostate cancer for the first time.<h4>Methods</h4>Reciprocal interactions between PC3 prostate cancer cells co-cultured with WPMY-1 prostatic fibroblasts were characterised using CTAP-MS. Signalling network changes were determined using Metascape and Enrichr and visualised using Cytoscape. Thymosin β4"],"journal":["Cellular oncology (Dordrecht, Netherlands)"],"pagination":["1311-1328"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9747870"],"repository":["biostudies-literature"],"pubmed_title":["Proteomic characterisation of prostate cancer intercellular communication reveals cell type-selective signalling and TMSB4X-dependent fibroblast reprogramming."],"pmcid":["PMC9747870"],"pubmed_authors":["Horvath LG","Nguyen EV","Taylor RA","Niranjan B","Daly RJ","Clark KC","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic characterisation of prostate cancer intercellular communication reveals cell type-selective signalling and TMSB4X-dependent fibroblast reprogramming.","description":"<h4>Background</h4>In prostate cancer, the tumour microenvironment (TME) represents an important regulator of disease progression and response to treatment. In the TME, cancer-associated fibroblasts (CAFs) play a key role in tumour progression, however the mechanisms underpinning fibroblast-cancer cell interactions are incompletely resolved. Here, we address this by applying cell type-specific labelling with amino acid precursors (CTAP) and mass spectrometry (MS)-based (phospho)proteomics to prostate cancer for the first time.<h4>Methods</h4>Reciprocal interactions between PC3 prostate cancer cells co-cultured with WPMY-1 prostatic fibroblasts were characterised using CTAP-MS. Signalling network changes were determined using Metascape and Enrichr and visualised using Cytoscape. Thymosin β4","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-07-14T15:28:49.435Z","creation":"2025-04-07T07:04:04.31Z"},"accession":"S-EPMC9747870","cross_references":{"pubmed":["36169805"],"doi":["10.1007/s13402-022-00719-z"]}}