biostudies-literatureGuo XTranslational Medicine Cross Research Fund of Shanghai Jiao Tong UniversityClinical Research Plan of Shanghai Hospital Development CenterShanghai Collaborative Innovation Center for Translational Medicine FundNational Natural Science Foundation of China531https://www.ebi.ac.uk/biostudies/studies/S-EPMC9749152biostudies-literatureUnknown20(1)Acute myocardial infarction (AMI) is usually caused by coronary thrombosis. However, the short half-life, lack of targetability and inevitable ischemia/reperfusion injury secondary to revascularization, which characterizes tissue plasminogen activator (tPA) limit its thrombolytic efficacy for AMI. To address the targeted and site-specific delivery of tPA, the current study reports the construction of a thrombus-targeting and responsive biomimetic nanoparticle (PTPN) for spatiotemporal treatment of AMI. PTPN was constituted by the thrombus microenvironment- responsive phenylboronic acid (PBA) nanocarrier, antioxidant molecular protocatechualdehyde (PC) and tPA with thrombolytic effect, which were enclosed by the platelet membrane. The thrombus-targeting capability of the platelet membrane eJournal of nanobiotechnologyThrombus-specific/responsive biomimetic nanomedicine for spatiotemporal thrombolysis and alleviation of myocardial ischemia/reperfusion injury.PMC9749152NO.TM202013No.SHDC2020CR3084BNo.YG2021ZD27, YG2022QN101 and YG2022ZD025Nos.81901419, 82072199 and 82172168Zang JGuo XHong TShao RHou XHe BWang KLiu WSu FfalseThrombus-specific/responsive biomimetic nanomedicine for spatiotemporal thrombolysis and alleviation of myocardial ischemia/reperfusion injury.Acute myocardial infarction (AMI) is usually caused by coronary thrombosis. However, the short half-life, lack of targetability and inevitable ischemia/reperfusion injury secondary to revascularization, which characterizes tissue plasminogen activator (tPA) limit its thrombolytic efficacy for AMI. To address the targeted and site-specific delivery of tPA, the current study reports the construction of a thrombus-targeting and responsive biomimetic nanoparticle (PTPN) for spatiotemporal treatment of AMI. PTPN was constituted by the thrombus microenvironment- responsive phenylboronic acid (PBA) nanocarrier, antioxidant molecular protocatechualdehyde (PC) and tPA with thrombolytic effect, which were enclosed by the platelet membrane. The thrombus-targeting capability of the platelet membrane e2022-01-01T00:00:00Z2022 Dec2025-04-05T15:42:27.004Z2025-04-05T15:42:27.004ZS-EPMC97491523651415410.1186/s12951-022-01686-1