biostudies-literatureWang SHMinistry of Science and Technology, TaiwanChang Gung Memorial Hospital, Linkou105https://www.ebi.ac.uk/biostudies/studies/S-EPMC9753400biostudies-literatureUnknown29(1)<h4>Background</h4>In endothelial cells, phospholipase C (PLC) β1-activated Ca²⁺ is a crucial second messenger for the signaling pathways governing angiogenesis. PLCβ1 is inactivated by complexing with an intracellular protein called translin-associated factor X (TRAX). This study demonstrates specific interactions between Globo H ceramide (GHCer) and TRAX, which highlight a new angiogenic control through PLCβ1 activation.<h4>Methods</h4>Globo-series glycosphingolipids (GSLs), including GHCer and stage-specific embryonic antigen-3 ceramide (SSEA3Cer), were analyzed using enzyme-linked immunosorbent assay (ELISA) and Biacore for their binding with TRAX. Angiogenic activities of GSLs in human umbilical vein endothelial cells (HUVECs) were evaluated. Molecular dynamics (MD) simulatJournal of biomedical scienceConformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis.PMC9753400CIRPG3L0011MOST 111-2321-B-182A-002OMRPG3C0047MOST 108-2321-B-182A-004MOST 109-2321-B-182A-005Hung JTHo YHWang SHKuo HHYu ALLin CCLee CWTsai HHCheng JYYu JLo TCfalseConformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis.<h4>Background</h4>In endothelial cells, phospholipase C (PLC) β1-activated Ca²⁺ is a crucial second messenger for the signaling pathways governing angiogenesis. PLCβ1 is inactivated by complexing with an intracellular protein called translin-associated factor X (TRAX). This study demonstrates specific interactions between Globo H ceramide (GHCer) and TRAX, which highlight a new angiogenic control through PLCβ1 activation.<h4>Methods</h4>Globo-series glycosphingolipids (GSLs), including GHCer and stage-specific embryonic antigen-3 ceramide (SSEA3Cer), were analyzed using enzyme-linked immunosorbent assay (ELISA) and Biacore for their binding with TRAX. Angiogenic activities of GSLs in human umbilical vein endothelial cells (HUVECs) were evaluated. Molecular dynamics (MD) simulat2022-01-01T00:00:00Z2022 Dec2026-05-28T05:37:00.419Z2025-04-05T15:42:58.392ZS-EPMC97534003651780610.1186/s12929-022-00889-w