{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hartford CCR"],"funding":["HHS | NIH | National Cancer Institute","HHS | NIH | National Cancer Institute (NCI)"],"pagination":["e0028922"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9753727"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(12)"],"pubmed_abstract":["<i>PURPL</i> is a p53-induced lncRNA that suppresses basal p53 levels. Here, we investigated <i>PURPL</i> upon p53 activation in liver cancer cells, where it is expressed at significantly higher levels than other cell types. Using isoform sequencing, we discovered novel <i>PURPL</i> transcripts that have a retained intron and/or previously unannotated exons. To determine <i>PURPL</i> function upon p53 activation, we performed transcriptome sequencing (RNA-Seq) after depleting <i>PURPL</i> using CRISPR interference (CRISPRi), followed by Nutlin treatment to induce p53. Strikingly, although loss of <i>PURPL</i> in untreated cells altered the expression of only 7 genes, loss of <i>PURPL</i> resulted in altered expression of ~800 genes upon p53 activation, revealing a context-dependent function of <i>PURPL</i>. Pathway analysis suggested that <i>PURPL</i> is important for fine-tuning the expression of specific genes required for mitosis. Consistent with these results, we observed a significant decrease in the percentage of mitotic cells upon <i>PURPL</i> depletion. Collectively, these data identify novel transcripts from the <i>PURPL</i> locus and suggest that <i>PURPL</i> delicately moderates the expression of mitotic genes in the context of p53 activation to control cell cycle arrest."],"journal":["Molecular and cellular biology"],"pubmed_title":["Context-Dependent Function of Long Noncoding RNA <i>PURPL</i> in Transcriptome Regulation during p53 Activation."],"pmcid":["PMC9753727"],"funding_grant_id":["ZIA BC 011646"],"pubmed_authors":["Chari R","Pongor L","Pasterczyk KR","Lal A","Chaudhary R","Gorospe M","Grammatikakis I","Shrestha RL","Li XL","Muys BR","Zhao Y","Fromont C","Kumar R","Aladjem MI","Hartford CCR","Khan J","Chen X","Tsitsipatis D","Basrai MA"],"additional_accession":[]},"is_claimable":false,"name":"Context-Dependent Function of Long Noncoding RNA <i>PURPL</i> in Transcriptome Regulation during p53 Activation.","description":"<i>PURPL</i> is a p53-induced lncRNA that suppresses basal p53 levels. Here, we investigated <i>PURPL</i> upon p53 activation in liver cancer cells, where it is expressed at significantly higher levels than other cell types. Using isoform sequencing, we discovered novel <i>PURPL</i> transcripts that have a retained intron and/or previously unannotated exons. To determine <i>PURPL</i> function upon p53 activation, we performed transcriptome sequencing (RNA-Seq) after depleting <i>PURPL</i> using CRISPR interference (CRISPRi), followed by Nutlin treatment to induce p53. Strikingly, although loss of <i>PURPL</i> in untreated cells altered the expression of only 7 genes, loss of <i>PURPL</i> resulted in altered expression of ~800 genes upon p53 activation, revealing a context-dependent function of <i>PURPL</i>. Pathway analysis suggested that <i>PURPL</i> is important for fine-tuning the expression of specific genes required for mitosis. Consistent with these results, we observed a significant decrease in the percentage of mitotic cells upon <i>PURPL</i> depletion. Collectively, these data identify novel transcripts from the <i>PURPL</i> locus and suggest that <i>PURPL</i> delicately moderates the expression of mitotic genes in the context of p53 activation to control cell cycle arrest.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-06-13T03:29:09.294Z","creation":"2025-02-19T02:37:55.857Z"},"accession":"S-EPMC9753727","cross_references":{"pubmed":["36342127"],"doi":["10.1128/mcb.00289-22"]}}