biostudies-literatureUnknown13An N<h4>Background</h4>Type I interferon (IFN) inhibits virus infection through multiple processes. Recent evidence indicates that IFN carries out its antiviral activity through readjusting of the cellular metabolism. The sterile alpha motif and histidine-aspartate domain containing protein 1 (SAMHD1), as an interferon-stimulated gene (ISG), has been reported to inhibit a number of retroviruses and DNA viruses, by depleting dNTPs indispensable for viral DNA replication. Here we report a new antiviral activity of SAMHD1 against RNA viruses including HCV and some other flaviviruses infection.<h4>Methods</h4>Multiple cellular and molecular biological technologies have been used to detect virus infection, replication and variation of intracellular proteins, including western blotting, qRT-PCR, GenFrontiers in immunology1007718https://www.ebi.ac.uk/biostudies/studies/S-EPMC9755837biostudies-literatureInterferon-inducible SAMHD1 restricts viral replication through downregulation of lipid synthesis.PMC9755837Liang CCen SLi XShao HAn NYang LGuo FLi QGe QYi DfalseInterferon-inducible SAMHD1 restricts viral replication through downregulation of lipid synthesis.<h4>Background</h4>Type I interferon (IFN) inhibits virus infection through multiple processes. Recent evidence indicates that IFN carries out its antiviral activity through readjusting of the cellular metabolism. The sterile alpha motif and histidine-aspartate domain containing protein 1 (SAMHD1), as an interferon-stimulated gene (ISG), has been reported to inhibit a number of retroviruses and DNA viruses, by depleting dNTPs indispensable for viral DNA replication. Here we report a new antiviral activity of SAMHD1 against RNA viruses including HCV and some other flaviviruses infection.<h4>Methods</h4>Multiple cellular and molecular biological technologies have been used to detect virus infection, replication and variation of intracellular proteins, including western blotting, qRT-PCR, Gen2022-01-01T00:00:00Z20222026-04-30T03:12:32.122Z2025-04-06T23:01:33.708ZS-EPMC97558373653207410.3389/fimmu.2022.1007718