{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kagamu H"],"funding":["Japan Agency for Medical Research and Development","Japan Society for the Promotion of Science"],"pagination":["4641-4653"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9755963"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["82(24)"],"pubmed_abstract":["CD4+ T-cell immunity helps clonal proliferation, migration, and cancer cell killing activity of CD8+ T cells and is essential in antitumor immune responses. To identify CD4+ T-cell clusters responsible for antitumor immunity, we simultaneously analyzed the naïve-effector state, Th polarization, and T-cell receptor clonotype based on single-cell RNA-sequencing data. Unsupervised clustering analysis uncovered the presence of a new CD4+ T-cell metacluster in the CD62Llow CD4+ T-cell subpopulation, which contained multicellular clonotypes associated with efficacy of programmed death-ligand 1 (PD-1) blockade therapy. The CD4+ T-cell metacluster consisted of CXCR3+CCR4-CCR6+ and CXCR3-CCR4-CCR6+ cells and was characterized by high expression of IL7 receptor and TCF7. The frequency of these cells"],"journal":["Cancer research"],"pubmed_title":["Single-Cell Analysis Reveals a CD4+ T-cell Cluster That Correlates with PD-1 Blockade Efficacy."],"pmcid":["PMC9755963"],"funding_grant_id":["19ae0101074h0001","17H04184"],"pubmed_authors":["Kagamu H","Yamasaki S","Mouri A","Imai H","Kanai Y","Kobayashi K","Yamaguchi O","Miura Y","Shibata T","Shiono A","Kaira K","Kitano S","Hashimoto K","Horimoto K","Nishihara F"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell Analysis Reveals a CD4+ T-cell Cluster That Correlates with PD-1 Blockade Efficacy.","description":"CD4+ T-cell immunity helps clonal proliferation, migration, and cancer cell killing activity of CD8+ T cells and is essential in antitumor immune responses. To identify CD4+ T-cell clusters responsible for antitumor immunity, we simultaneously analyzed the naïve-effector state, Th polarization, and T-cell receptor clonotype based on single-cell RNA-sequencing data. Unsupervised clustering analysis uncovered the presence of a new CD4+ T-cell metacluster in the CD62Llow CD4+ T-cell subpopulation, which contained multicellular clonotypes associated with efficacy of programmed death-ligand 1 (PD-1) blockade therapy. The CD4+ T-cell metacluster consisted of CXCR3+CCR4-CCR6+ and CXCR3-CCR4-CCR6+ cells and was characterized by high expression of IL7 receptor and TCF7. The frequency of these cells","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-07-14T21:12:14.1Z","creation":"2025-04-06T14:10:18.263Z"},"accession":"S-EPMC9755963","cross_references":{"pubmed":["36219677"],"doi":["10.1158/0008-5472.CAN-22-0112"]}}