biostudies-literatureUnknown13Chen XAnisakidosis is a food-borne parasitic disease (FBPD) caused by the third-stage larvae of the family Anisakidae. Therefore, it is important to develop a simple, rapid and equipment-free detection method for anisakids in fish samples or seafood since current methods are time-consuming and require complex instruments. In this study, a recombinase polymerase amplification (RPA)-based method was established for the first time to detect anisakids by targeting the internal transcribed spacer (ITS) regions. The detection results were visualized by including SYBR Green I (SG) in the method. The sensitivity of RPA-SG assay was 10² copies per reaction of recombinant plasmid (within 20 min at 37°C), similar to quantitative real-time PCR (qPCR). The assay had high specificity for detecting Frontiers in microbiology1026129https://www.ebi.ac.uk/biostudies/studies/S-EPMC9756439biostudies-literatureRapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I.PMC9756439Qiu YZhao LLiu MDong ZChen XWang HWang XZhang CWang JBai XfalseRapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I.Anisakidosis is a food-borne parasitic disease (FBPD) caused by the third-stage larvae of the family Anisakidae. Therefore, it is important to develop a simple, rapid and equipment-free detection method for anisakids in fish samples or seafood since current methods are time-consuming and require complex instruments. In this study, a recombinase polymerase amplification (RPA)-based method was established for the first time to detect anisakids by targeting the internal transcribed spacer (ITS) regions. The detection results were visualized by including SYBR Green I (SG) in the method. The sensitivity of RPA-SG assay was 10² copies per reaction of recombinant plasmid (within 20 min at 37°C), similar to quantitative real-time PCR (qPCR). The assay had high specificity for detecting 2022-01-01T00:00:00Z20222025-04-04T12:01:48.469Z2025-02-19T03:00:11.917ZS-EPMC97564393653244710.3389/fmicb.2022.1026129