biostudies-literatureUnknownTrivedi SDepartment of Biology of Masaryk University, Faculty of MedicineCzech Science FoundationMeiotic chromosome segregation relies on programmed DNA double-strand break induction. These are in turn repaired by homologous recombination, generating physical attachments between the parental chromosomes called crossovers. A subset of breaks yields recombinant outcomes, while crossover-independent mechanisms repair the majority of lesions. The balance between different repair pathways is crucial to ensure genome integrity. We show that Caenorhabditis elegans BRC-1/BRCA1-BRD-1/BARD1 and PARG-1/PARG form a complex in vivo, essential for accurate DNA repair in the germline. Simultaneous depletion of BRC-1 and PARG-1 causes synthetic lethality due to reduced crossover formation and impaired break repair, evidenced by hindered RPA-1 removal and presence of aberrant chromatin bodies in diakiNucleic acids researchgkac1153https://www.ebi.ac.uk/biostudies/studies/S-EPMC9757042biostudies-literaturePARG and BRCA1-BARD1 cooperative function regulates DNA repair pathway choice during gametogenesis.PMC9757042GA20-08819STrivedi SBlazickova JSilva NfalsePARG and BRCA1-BARD1 cooperative function regulates DNA repair pathway choice during gametogenesis.Meiotic chromosome segregation relies on programmed DNA double-strand break induction. These are in turn repaired by homologous recombination, generating physical attachments between the parental chromosomes called crossovers. A subset of breaks yields recombinant outcomes, while crossover-independent mechanisms repair the majority of lesions. The balance between different repair pathways is crucial to ensure genome integrity. We show that Caenorhabditis elegans BRC-1/BRCA1-BRD-1/BARD1 and PARG-1/PARG form a complex in vivo, essential for accurate DNA repair in the germline. Simultaneous depletion of BRC-1 and PARG-1 causes synthetic lethality due to reduced crossover formation and impaired break repair, evidenced by hindered RPA-1 removal and presence of aberrant chromatin bodies in diaki2022-01-01T00:00:00Z2022 Dec2025-04-04T08:56:00.818Z2025-04-04T08:56:00.818ZS-EPMC97570423647809710.1093/nar/gkac1153