biostudies-literatureGomez-Linan CSpanish Ministry of Science and InnovationSpanish National Research CouncilLa Caixa Foundation-Health Research 2020 Call12251-12265https://www.ebi.ac.uk/biostudies/studies/S-EPMC9757043biostudies-literatureUnknown50(21)In-depth analysis of the transcriptomes of several model organisms has revealed that genomes are pervasively transcribed, giving rise to an abundance of non-canonical and mainly antisense RNA polymerase II-derived transcripts that are produced from almost any genomic context. Pervasive RNAs are degraded by surveillance mechanisms, but the repertoire of proteins that control the fate of these non-productive transcripts is still incomplete. Trypanosomes are single-celled eukaryotes that show constitutive RNA polymerase II transcription and in which initiation and termination of transcription occur at a limited number of sites per chromosome. It is not known whether pervasive transcription exists in organisms with unregulated RNA polymerase II activity, and which factors could be involved in Nucleic acids researchThe RNA-binding protein RBP33 dampens non-productive transcription in trypanosomes.PMC9757043201920E114PID2019-111109RB-I00HR20-00635Gomez-Linan CCeballos-Perez GFernandez-Moya SMEstevez AMGomez-Diaz EfalseThe RNA-binding protein RBP33 dampens non-productive transcription in trypanosomes.In-depth analysis of the transcriptomes of several model organisms has revealed that genomes are pervasively transcribed, giving rise to an abundance of non-canonical and mainly antisense RNA polymerase II-derived transcripts that are produced from almost any genomic context. Pervasive RNAs are degraded by surveillance mechanisms, but the repertoire of proteins that control the fate of these non-productive transcripts is still incomplete. Trypanosomes are single-celled eukaryotes that show constitutive RNA polymerase II transcription and in which initiation and termination of transcription occur at a limited number of sites per chromosome. It is not known whether pervasive transcription exists in organisms with unregulated RNA polymerase II activity, and which factors could be involved in 2022-01-01T00:00:00Z2022 Nov2026-05-08T03:15:02.98Z2025-06-01T01:56:23.003ZS-EPMC97570433645400810.1093/nar/gkac1123