{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Erkelens AM"],"funding":["China Scholarship Council","Dutch Research Council (NWO)","Ministry of Science and Innovation of the Spanish Government","Spanish Ministry of Universities and Autonomous University of Madrid"],"pagination":["12166-12185"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9757077"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(21)"],"pubmed_abstract":["Nucleoid-associated proteins (NAPs) play a central role in chromosome organization and environment-responsive transcription regulation. The Bacillus subtilis-encoded NAP Rok binds preferentially AT-rich regions of the genome, which often contain genes of foreign origin that are silenced by Rok binding. Additionally, Rok plays a role in chromosome architecture by binding in genomic clusters and promoting chromosomal loop formation. Based on this, Rok was proposed to be a functional homolog of E. coli H-NS. However, it is largely unclear how Rok binds DNA, how it represses transcription and whether Rok mediates environment-responsive gene regulation. Here, we investigated Rok's DNA binding properties and the effects of physico-chemical conditions thereon. We demonstrate that Rok is a DNA bri"],"journal":["Nucleic acids research"],"pubmed_title":["The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues."],"pmcid":["PMC9757077"],"funding_grant_id":["PID2019_108778GB_C21","201506880001","016.160.613","VICI 016.160.613/533","OCENW.GROOT.2019.012"],"pubmed_authors":["Erkelens AM","Keek HGJ","Dame RT","Miguel-Arribas A","Qin L","Markus D","Meijer WJJ","Abia D","Cajili MKM","van Erp B","Schwab S"],"additional_accession":[]},"is_claimable":false,"name":"The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues.","description":"Nucleoid-associated proteins (NAPs) play a central role in chromosome organization and environment-responsive transcription regulation. The Bacillus subtilis-encoded NAP Rok binds preferentially AT-rich regions of the genome, which often contain genes of foreign origin that are silenced by Rok binding. Additionally, Rok plays a role in chromosome architecture by binding in genomic clusters and promoting chromosomal loop formation. Based on this, Rok was proposed to be a functional homolog of E. coli H-NS. However, it is largely unclear how Rok binds DNA, how it represses transcription and whether Rok mediates environment-responsive gene regulation. Here, we investigated Rok's DNA binding properties and the effects of physico-chemical conditions thereon. We demonstrate that Rok is a DNA bri","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-06-22T03:14:08.671Z","creation":"2024-11-06T22:12:52.066Z"},"accession":"S-EPMC9757077","cross_references":{"pubmed":["36408910"],"doi":["10.1093/nar/gkac1064"]}}