biostudies-literatureUnknown10Zhao YPhosphorus starvation response (PHR) protein is an important transcription factor in phosphorus regulatory network, which plays a vital role in regulating the effective utilization of phosphorus. So far, the <i>PHR</i> genes have not been systematically investigated in cotton. In the present study, we have identified 22, 23, 41 and 42 <i>PHR</i> genes in <i>G. arboreum</i>, <i>G. raimondii</i>, <i>G. hirsutum</i> and <i>G. barbadense</i>, respectively. Phylogenetic analysis showed that cotton <i>PHR</i> genes were classified into five distinct subfamilies. The gene structure, protein motifs and gene expression were further investigated. The <i>PHR</i> genes of <i>G. hirsutum</i> from the same subfamily had similar gene structures, all containing Myb_DNA-binding and Myb_CC_LHEQLE conserved domain. The structures of paralogous genes were considerably conserved in exons number and introns length. The cis-element prediction in their promoters showed that genes were not only regulated by light induction, but also were related to auxin, MeJA, abscisic acid-responsive elements, of which might be regulated by miRNA. The expression analysis showed that the <i>GhPHR</i> genes were differentially expressed in different tissues under various stresses. Furthermore, <i>GhPHR6</i>, <i>GhPHR11</i>, <i>GhPHR18</i> and <i>GhPHR38</i> were significantly changed under low phosphorus stress. The results of this study provide a basis for further cloning and functional verification of genes related to regulatory network of low phosphorus tolerance in cotton.PeerJe14584https://www.ebi.ac.uk/biostudies/studies/S-EPMC9760022biostudies-literatureGenome-wide investigation and expression pattern of <i>PHR</i> family genes in cotton under low phosphorus stress.PMC9760022Li YFeng JGuo YWang SLi PWang HSu YLi LZhao YSun ZfalseGenome-wide investigation and expression pattern of <i>PHR</i> family genes in cotton under low phosphorus stress.Phosphorus starvation response (PHR) protein is an important transcription factor in phosphorus regulatory network, which plays a vital role in regulating the effective utilization of phosphorus. So far, the <i>PHR</i> genes have not been systematically investigated in cotton. In the present study, we have identified 22, 23, 41 and 42 <i>PHR</i> genes in <i>G. arboreum</i>, <i>G. raimondii</i>, <i>G. hirsutum</i> and <i>G. barbadense</i>, respectively. Phylogenetic analysis showed that cotton <i>PHR</i> genes were classified into five distinct subfamilies. The gene structure, protein motifs and gene expression were further investigated. The <i>PHR</i> genes of <i>G. hirsutum</i> from the same subfamily had similar gene structures, all containing Myb_DNA-binding and Myb_CC_LHEQLE conserved domain. The structures of paralogous genes were considerably conserved in exons number and introns length. The cis-element prediction in their promoters showed that genes were not only regulated by light induction, but also were related to auxin, MeJA, abscisic acid-responsive elements, of which might be regulated by miRNA. The expression analysis showed that the <i>GhPHR</i> genes were differentially expressed in different tissues under various stresses. Furthermore, <i>GhPHR6</i>, <i>GhPHR11</i>, <i>GhPHR18</i> and <i>GhPHR38</i> were significantly changed under low phosphorus stress. The results of this study provide a basis for further cloning and functional verification of genes related to regulatory network of low phosphorus tolerance in cotton.2022-01-01T00:00:00Z20222026-05-14T03:11:28.738Z2025-06-01T02:49:11.863ZS-EPMC97600223654080610.7717/peerj.14584