{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Yang J"],"pubmed_abstract":["<h4>Introduction</h4>A facultative anaerobe bacterium <i>Shewanella xiamenensis</i> CQ-Y1 was isolated from the wastewater of Changqing oilfield in Shaanxi Province of China. <i>Shewanella</i> is the important dissimilatory metal-reducing bacteria. It exhibited a well potential application in biodegradation and bioremediation.<h4>Methods</h4>Genome sequencing, assembling and functional annotation were conducted to explore the genome information of CQ-Y1. The effect of temperatures and NaCl concentrations on the CQ-Y1 growth and Fe(III) reduction were investigated by UV visible spectrophotometry, SEM and XRD.<h4>Results</h4>Genomic analysis revealed its complete genome was a circular chromosome of 4,710,887 bp with a GC content of 46.50% and 4,110 CDSs genes, 86 tRNAs and 26 rRNAs. It contains genes encoding for Na<sup>+</sup>/H<sup>+</sup> antiporter, K<sup>+</sup>/Cl<sup>-</sup> transporter, heat shock protein associated with NaCl and high-temperature resistance. The presence of genes related to flavin, Cytochrome <i>c</i>, siderophore, and other related proteins supported Fe(III) reduction. In addition, CQ-Y1 could survive at 10% NaCl (w/v) and 45°C, and temperature showed more pronounced effects than NaCl concentration on the bacterial growth. The maximum Fe(III) reduction ratio of CQ-Y1 reached 70.1% at 30°C without NaCl, and the reduction reaction remained active at 40°C with 3% NaCl (w/v). NaCl concentration was more effective than temperature on microbial Fe(III) reduction. And the reduction products under high temperature and high NaCl conditions were characterized as Fe<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, FeCl<sub>2</sub> and Fe(OH)<sub>2</sub>.<h4>Discussion</h4>Accordingly, a Fe(III) reduction mechanism of CQ-Y1 mediated by Cytochrome <i>c</i> and flavin was hypothesised. These findings could provide information for a better understanding of the origin and evolution of genomic and metabolic diversity of <i>S. xiamenensis</i>."],"journal":["Frontiers in microbiology"],"pagination":["1028030"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9760863"],"repository":["biostudies-literature"],"pubmed_title":["Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant <i>Shewanella xiamenensis</i> from Changqing oilfield of China."],"pmcid":["PMC9760863"],"pubmed_authors":["Yan L","Yang J","Gu JD","Wang W","Zhao D","Liu T","Zhang S"],"additional_accession":[]},"is_claimable":false,"name":"Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant <i>Shewanella xiamenensis</i> from Changqing oilfield of China.","description":"<h4>Introduction</h4>A facultative anaerobe bacterium <i>Shewanella xiamenensis</i> CQ-Y1 was isolated from the wastewater of Changqing oilfield in Shaanxi Province of China. <i>Shewanella</i> is the important dissimilatory metal-reducing bacteria. It exhibited a well potential application in biodegradation and bioremediation.<h4>Methods</h4>Genome sequencing, assembling and functional annotation were conducted to explore the genome information of CQ-Y1. The effect of temperatures and NaCl concentrations on the CQ-Y1 growth and Fe(III) reduction were investigated by UV visible spectrophotometry, SEM and XRD.<h4>Results</h4>Genomic analysis revealed its complete genome was a circular chromosome of 4,710,887 bp with a GC content of 46.50% and 4,110 CDSs genes, 86 tRNAs and 26 rRNAs. It contains genes encoding for Na<sup>+</sup>/H<sup>+</sup> antiporter, K<sup>+</sup>/Cl<sup>-</sup> transporter, heat shock protein associated with NaCl and high-temperature resistance. The presence of genes related to flavin, Cytochrome <i>c</i>, siderophore, and other related proteins supported Fe(III) reduction. In addition, CQ-Y1 could survive at 10% NaCl (w/v) and 45°C, and temperature showed more pronounced effects than NaCl concentration on the bacterial growth. The maximum Fe(III) reduction ratio of CQ-Y1 reached 70.1% at 30°C without NaCl, and the reduction reaction remained active at 40°C with 3% NaCl (w/v). NaCl concentration was more effective than temperature on microbial Fe(III) reduction. And the reduction products under high temperature and high NaCl conditions were characterized as Fe<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, FeCl<sub>2</sub> and Fe(OH)<sub>2</sub>.<h4>Discussion</h4>Accordingly, a Fe(III) reduction mechanism of CQ-Y1 mediated by Cytochrome <i>c</i> and flavin was hypothesised. These findings could provide information for a better understanding of the origin and evolution of genomic and metabolic diversity of <i>S. xiamenensis</i>.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-28T20:57:25.212Z","creation":"2025-02-19T03:00:02.252Z"},"accession":"S-EPMC9760863","cross_references":{"pubmed":["36545192"],"doi":["10.3389/fmicb.2022.1028030"]}}