biostudies-literatureUnknown13Yang J<h4>Introduction</h4>A facultative anaerobe bacterium <i>Shewanella xiamenensis</i> CQ-Y1 was isolated from the wastewater of Changqing oilfield in Shaanxi Province of China. <i>Shewanella</i> is the important dissimilatory metal-reducing bacteria. It exhibited a well potential application in biodegradation and bioremediation.<h4>Methods</h4>Genome sequencing, assembling and functional annotation were conducted to explore the genome information of CQ-Y1. The effect of temperatures and NaCl concentrations on the CQ-Y1 growth and Fe(III) reduction were investigated by UV visible spectrophotometry, SEM and XRD.<h4>Results</h4>Genomic analysis revealed its complete genome was a circular chromosome of 4,710,887 bp with a GC content of 46.50% and 4,110 CDSs genes, 86 tRNAs and 26 rRNAs. It contains genes encoding for Na⁺/H⁺ antiporter, K⁺/Cl^- transporter, heat shock protein associated with NaCl and high-temperature resistance. The presence of genes related to flavin, Cytochrome <i>c</i>, siderophore, and other related proteins supported Fe(III) reduction. In addition, CQ-Y1 could survive at 10% NaCl (w/v) and 45°C, and temperature showed more pronounced effects than NaCl concentration on the bacterial growth. The maximum Fe(III) reduction ratio of CQ-Y1 reached 70.1% at 30°C without NaCl, and the reduction reaction remained active at 40°C with 3% NaCl (w/v). NaCl concentration was more effective than temperature on microbial Fe(III) reduction. And the reduction products under high temperature and high NaCl conditions were characterized as Fe₃(PO₄)₂, FeCl₂ and Fe(OH)₂.<h4>Discussion</h4>Accordingly, a Fe(III) reduction mechanism of CQ-Y1 mediated by Cytochrome <i>c</i> and flavin was hypothesised. These findings could provide information for a better understanding of the origin and evolution of genomic and metabolic diversity of <i>S. xiamenensis</i>.Frontiers in microbiology1028030https://www.ebi.ac.uk/biostudies/studies/S-EPMC9760863biostudies-literatureGrowth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant <i>Shewanella xiamenensis</i> from Changqing oilfield of China.PMC9760863Yan LYang JGu JDWang WZhao DLiu TZhang SfalseGrowth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerant <i>Shewanella xiamenensis</i> from Changqing oilfield of China.<h4>Introduction</h4>A facultative anaerobe bacterium <i>Shewanella xiamenensis</i> CQ-Y1 was isolated from the wastewater of Changqing oilfield in Shaanxi Province of China. <i>Shewanella</i> is the important dissimilatory metal-reducing bacteria. It exhibited a well potential application in biodegradation and bioremediation.<h4>Methods</h4>Genome sequencing, assembling and functional annotation were conducted to explore the genome information of CQ-Y1. The effect of temperatures and NaCl concentrations on the CQ-Y1 growth and Fe(III) reduction were investigated by UV visible spectrophotometry, SEM and XRD.<h4>Results</h4>Genomic analysis revealed its complete genome was a circular chromosome of 4,710,887 bp with a GC content of 46.50% and 4,110 CDSs genes, 86 tRNAs and 26 rRNAs. It contains genes encoding for Na⁺/H⁺ antiporter, K⁺/Cl^- transporter, heat shock protein associated with NaCl and high-temperature resistance. The presence of genes related to flavin, Cytochrome <i>c</i>, siderophore, and other related proteins supported Fe(III) reduction. In addition, CQ-Y1 could survive at 10% NaCl (w/v) and 45°C, and temperature showed more pronounced effects than NaCl concentration on the bacterial growth. The maximum Fe(III) reduction ratio of CQ-Y1 reached 70.1% at 30°C without NaCl, and the reduction reaction remained active at 40°C with 3% NaCl (w/v). NaCl concentration was more effective than temperature on microbial Fe(III) reduction. And the reduction products under high temperature and high NaCl conditions were characterized as Fe₃(PO₄)₂, FeCl₂ and Fe(OH)₂.<h4>Discussion</h4>Accordingly, a Fe(III) reduction mechanism of CQ-Y1 mediated by Cytochrome <i>c</i> and flavin was hypothesised. These findings could provide information for a better understanding of the origin and evolution of genomic and metabolic diversity of <i>S. xiamenensis</i>.2022-01-01T00:00:00Z20222026-05-28T20:57:25.212Z2025-02-19T03:00:02.252ZS-EPMC97608633654519210.3389/fmicb.2022.1028030