{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Zhao G"],"pubmed_abstract":["<h4>Introduction</h4>Pancreatic adenocarcinoma (PAAD) is a fatal disease characterized by promoting connective tissue proliferation in the stroma. Activated cancer-associated fibroblasts (CAFs) play a key role in fibrogenesis in PAAD. CAF-based tumor typing of PAAD has not been explored.<h4>Methods</h4>We extracted single-cell sequence transcriptomic data from GSE154778 and CRA001160 datasets from Gene Expression Omnibus or Tumor Immune Single-cell Hub to collect CAFs in PAAD. On the basis of Seurat packages and new algorithms in machine learning, CAF-related subtypes and their top genes for PAAD were analyzed and visualized. We used CellChat package to perform cell-cell communication analysis. In addition, we carried out functional enrichment analysis based on clusterProfiler package. Fin"],"journal":["Frontiers in oncology"],"pagination":["1045477"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9762551"],"repository":["biostudies-literature"],"pubmed_title":["The novel subclusters based on cancer-associated fibroblast for pancreatic adenocarcinoma."],"pmcid":["PMC9762551"],"pubmed_authors":["Zhang W","Yang H","Wang C","Zhao G","Jiao J"],"additional_accession":[]},"is_claimable":false,"name":"The novel subclusters based on cancer-associated fibroblast for pancreatic adenocarcinoma.","description":"<h4>Introduction</h4>Pancreatic adenocarcinoma (PAAD) is a fatal disease characterized by promoting connective tissue proliferation in the stroma. Activated cancer-associated fibroblasts (CAFs) play a key role in fibrogenesis in PAAD. CAF-based tumor typing of PAAD has not been explored.<h4>Methods</h4>We extracted single-cell sequence transcriptomic data from GSE154778 and CRA001160 datasets from Gene Expression Omnibus or Tumor Immune Single-cell Hub to collect CAFs in PAAD. On the basis of Seurat packages and new algorithms in machine learning, CAF-related subtypes and their top genes for PAAD were analyzed and visualized. We used CellChat package to perform cell-cell communication analysis. In addition, we carried out functional enrichment analysis based on clusterProfiler package. Fin","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-05-29T20:28:52.349Z","creation":"2024-11-07T01:34:33.828Z"},"accession":"S-EPMC9762551","cross_references":{"pubmed":["36544710"],"doi":["10.3389/fonc.2022.1045477"]}}