biostudies-literatureUnknown12Zhao G<h4>Introduction</h4>Pancreatic adenocarcinoma (PAAD) is a fatal disease characterized by promoting connective tissue proliferation in the stroma. Activated cancer-associated fibroblasts (CAFs) play a key role in fibrogenesis in PAAD. CAF-based tumor typing of PAAD has not been explored.<h4>Methods</h4>We extracted single-cell sequence transcriptomic data from GSE154778 and CRA001160 datasets from Gene Expression Omnibus or Tumor Immune Single-cell Hub to collect CAFs in PAAD. On the basis of Seurat packages and new algorithms in machine learning, CAF-related subtypes and their top genes for PAAD were analyzed and visualized. We used CellChat package to perform cell-cell communication analysis. In addition, we carried out functional enrichment analysis based on clusterProfiler package. FinFrontiers in oncology1045477https://www.ebi.ac.uk/biostudies/studies/S-EPMC9762551biostudies-literatureThe novel subclusters based on cancer-associated fibroblast for pancreatic adenocarcinoma.PMC9762551Zhang WYang HWang CZhao GJiao JfalseThe novel subclusters based on cancer-associated fibroblast for pancreatic adenocarcinoma.<h4>Introduction</h4>Pancreatic adenocarcinoma (PAAD) is a fatal disease characterized by promoting connective tissue proliferation in the stroma. Activated cancer-associated fibroblasts (CAFs) play a key role in fibrogenesis in PAAD. CAF-based tumor typing of PAAD has not been explored.<h4>Methods</h4>We extracted single-cell sequence transcriptomic data from GSE154778 and CRA001160 datasets from Gene Expression Omnibus or Tumor Immune Single-cell Hub to collect CAFs in PAAD. On the basis of Seurat packages and new algorithms in machine learning, CAF-related subtypes and their top genes for PAAD were analyzed and visualized. We used CellChat package to perform cell-cell communication analysis. In addition, we carried out functional enrichment analysis based on clusterProfiler package. Fin2022-01-01T00:00:00Z20222025-05-29T20:28:52.349Z2024-11-07T01:34:33.828ZS-EPMC97625513654471010.3389/fonc.2022.1045477