{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Croft CL"],"funding":["Alzheimer's Research UK (ARUK)","U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging)"],"pagination":["173"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9763367"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["The accumulation of α-synuclein (α-syn) in intracellular formations known as Lewy bodies (LBs) is associated with several neurodegenerative diseases including Parkinson's disease and Lewy Body Dementia. There is still limited understanding of how α-syn and LB formation is associated with cellular dysfunction and degeneration in these diseases. To examine the clearance and production dynamics of α-syn we transduced organotypic murine brain slice cultures (BSCs) with recombinant adeno-associated viruses (rAAVs) to express Dendra2-tagged human wild-type (WT) and mutant A53T α-syn, with and without the addition of exogenous α-syn fibrillar seeds and tracked them over several weeks in culture using optical pulse labeling. We found that neurons expressing WT or mutant A53T human α-syn show simil"],"journal":["NPJ Parkinson's disease"],"pubmed_title":["Optical pulse labeling studies reveal exogenous seeding slows α-synuclein clearance."],"pmcid":["PMC9763367"],"funding_grant_id":["U01AG046139","U01TR003715","ARUK-RADF2019A-003"],"pubmed_authors":["Goodwin MS","Moran CA","Paterno G","Cruz PE","Giasson BI","Croft CL","Ryu DH","Vause AR","Rowe LA","Golde TE"],"additional_accession":[]},"is_claimable":false,"name":"Optical pulse labeling studies reveal exogenous seeding slows α-synuclein clearance.","description":"The accumulation of α-synuclein (α-syn) in intracellular formations known as Lewy bodies (LBs) is associated with several neurodegenerative diseases including Parkinson's disease and Lewy Body Dementia. There is still limited understanding of how α-syn and LB formation is associated with cellular dysfunction and degeneration in these diseases. To examine the clearance and production dynamics of α-syn we transduced organotypic murine brain slice cultures (BSCs) with recombinant adeno-associated viruses (rAAVs) to express Dendra2-tagged human wild-type (WT) and mutant A53T α-syn, with and without the addition of exogenous α-syn fibrillar seeds and tracked them over several weeks in culture using optical pulse labeling. We found that neurons expressing WT or mutant A53T human α-syn show simil","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-26T09:49:07.388Z","creation":"2025-04-06T13:08:22.064Z"},"accession":"S-EPMC9763367","cross_references":{"pubmed":["36535953"],"doi":["10.1038/s41531-022-00434-4"]}}